Carbonic anhydrase as a model for biophysical and physical-organic studies of proteins and protein− ligand binding

VM Krishnamurthy, GK Kaufman, AR Urbach… - Chemical …, 2008 - ACS Publications
Carbonic anhydrase (CA, EC 4.2. 1.1) is a protein that is especially well-suited to serve as a
model in many types of studies in biophysics, bioanalysis, the physical-organic chemistry of …

Why are proteins charged? Networks of charge–charge interactions in proteins measured by charge ladders and capillary electrophoresis

I Gitlin, JD Carbeck… - Angewandte Chemie …, 2006 - Wiley Online Library
Almost all proteins contain charged amino acids. While the function in catalysis or binding of
individual charges in the active site can often be identified, it is less clear how to assign …

Rapid prototyping of microfluidic systems in poly (dimethylsiloxane)

DC Duffy, JC McDonald, OJA Schueller… - Analytical …, 1998 - ACS Publications
This paper describes a procedure that makes it possible to design and fabricate (including
sealing) microfluidic systems in an elastomeric material poly (dimethylsiloxane)(PDMS) in …

Hydrogen bonds and salt bridges across protein-protein interfaces.

D Xu, CJ Tsai, R Nussinov - Protein engineering, 1997 - academic.oup.com
To understand further, and to utilize, the interactions across protein-protein interfaces, we
carried out an analysis of the hydrogen bonds and of the salt bridges in a collection of 319 …

A strategy for the generation of surfaces presenting ligands for studies of binding based on an active ester as a common reactive intermediate: a surface plasmon …

J Lahiri, L Isaacs, J Tien, GM Whitesides - Analytical chemistry, 1999 - ACS Publications
This paper describes the immobilization of ten proteins and two low-molecular-weight
ligands on mixed selfassembled monolayers (SAMs) of alkanethiolates on gold generated …

The interaction of proteins and cells with self-assembled monolayers of alkanethiolates on gold and silver

E Ostuni, L Yan, GM Whitesides - Colloids and Surfaces B: Biointerfaces, 1999 - Elsevier
Alkanethiols, HS (CH2) nX, chemisorb on gold and silver and form self-assembled
monolayers (SAMs). The ability to present a variety of functional groups, X, at the terminal …

[图书][B] Handbook of affinity chromatography

T Kline - 1993 - taylorfrancis.com
Outlining the fundamental principles by which all interactions occur, this reference focuses
on harnessing the biochemistry of bioorganic compounds in order to separate them …

Scaling form of viscosity at all length-scales in poly (ethylene glycol) solutions studied by fluorescence correlation spectroscopy and capillary electrophoresis

R Holyst, A Bielejewska, J Szymański, A Wilk… - Physical Chemistry …, 2009 - pubs.rsc.org
We measured the viscosity of poly (ethylene glycol)(PEG 6000, 12 000, 20 000) in water
using capillary electrophoresis and fluorescence correlation spectroscopy with nanoscopic …

Investigation of nonsynonymous mutations in the spike protein of SARS-CoV-2 and its interaction with the ACE2 receptor by molecular docking and MM/GBSA …

RY Aljindan, AM Al-Subaie, AI Al-Ohali… - Computers in biology and …, 2021 - Elsevier
COVID-19 is an infectious and pathogenic viral disease caused by SARS-CoV-2 that leads
to septic shock, coagulation dysfunction, and acute respiratory distress syndrome. The …

Glycosylation provides both stimulatory and inhibitory effects on cell surface and soluble CD44 binding to hyaluronan

TP Skelton, C Zeng, A Nocks… - The Journal of cell biology, 1998 - rupress.org
Glycosylation has been implicated in the regulation of CD44-mediated cell binding of
hyaluronan (HA). However, neither the relative contribution of N-and O-linked glycans nor …