The serine protease subtilisin is an important industrial enzyme as well as a model for
understanding the enormous rate enhancements affected by enzymes. For these reasons …

Encapsulation of biomolecules using metal–organic frameworks (MOFs) to form stable
biocomposites has been demonstrated to be a valuable strategy for their preservation and …

In this review, we discuss the progress in the study and modification of subtilisin proteases.
Despite longstanding applications of microbial proteases and a large number of research …

The gene encoding a subtilisin‐like serine proteinase in the psychrotrophic Vibrio sp. PA44
has been successfully cloned, sequenced and expressed in Escherichia coli. The gene is …

Pseudomonas fluorescens RO98, a raw milk isolate, was inoculated into McKellar's minimal
salts medium and incubated at 25° C for 48 h to allow production of protease. A zinc …

The investigation of the structural determinants of enzymatic temperature adaptation is a
crucial pre-requisite both in terms of fundamental research and industrial applications to …

Enzymes used during washing in laundry detergents have become a universal tool to lower
energy consumption and to generate a broad, consumer-relevant, cleaning effect. However …

Fluorescence depolarization and decay kinetic profiles, together with differential scanning
calorimetric thermograms and circular dichroism spectra, are measured to understand the …

The mature lactococcal cell envelope proteinase (CEP) consists of an N-terminal subtilisin-
like proteinase domain and a large C-terminal extension of unknown function whose far end …

Chelators are a common ingredient in most laundry detergents. They have a number of
different functions such as reducing water hardness, assisting in keeping particulate soil in …