Improved taxonomic assignment of human intestinal 16S rRNA sequences by a dedicated reference database

J Ritari, J Salojärvi, L Lahti, WM de Vos - BMC genomics, 2015 - Springer
Background Current sequencing technology enables taxonomic profiling of microbial
ecosystems at high resolution and depth by using the 16S rRNA gene as a phylogenetic …

Determining the reliability of measuring mercury cycling gene abundance with correlations with mercury and methylmercury concentrations

GA Christensen, CM Gionfriddo, AJ King… - … science & technology, 2019 - ACS Publications
Methylmercury (MeHg) is a bioaccumulative toxic contaminant in many ecosystems, but
factors governing its production are poorly understood. Recent work has shown that the …

Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each …

R Rosselli, O Romoli, N Vitulo, A Vezzi… - Scientific reports, 2016 - nature.com
The analysis of environmental microbial communities has largely relied on a PCR-
dependent amplification of genes entailing species identity as 16S rRNA. This approach is …

Archaeal and bacterial communities in three alkaline hot springs in Heart Lake Geyser Basin, Yellowstone National Park

K Bowen De León, R Gerlach, BM Peyton… - Frontiers in …, 2013 - frontiersin.org
The Heart Lake Geyser Basin (HLGB) is remotely located at the base of Mount Sheridan in
southern Yellowstone National Park (YNP), Wyoming, USA and is situated along Witch …

Environmental bio-monitoring with high-throughput sequencing

J Wang, PA McLenachan, PJ Biggs… - Briefings in …, 2013 - academic.oup.com
There is much interest in using high-throughput DNA sequencing methodology to monitor
microorganisms, complex plant and animal communities. However, there are experimental …

User‐friendly bioinformatics pipeline gDAT (graphical downstream analysis tool) for analysing rDNA sequences

M Vasar, J Davison, L Neuenkamp… - Molecular Ecology …, 2021 - Wiley Online Library
High‐throughput sequencing (HTS) of multiple organisms in parallel (metabarcoding) has
become a routine and cost‐effective method for the analysis of microbial communities in …

Phylogenetic affiliation of SSU rRNA genes generated by massively parallel sequencing: new insights into the freshwater protist diversity

N Taib, JF Mangot, I Domaizon, G Bronner… - PLoS One, 2013 - journals.plos.org
Recent advances in next-generation sequencing (NGS) technologies spur progress in
determining the microbial diversity in various ecosystems by highlighting, for example, the …

Investigation of coal-associated bacterial and archaeal populations from a diffusive microbial sampler (DMS)

EP Barnhart, KB De León, BD Ramsay… - International Journal of …, 2013 - Elsevier
Abstract The Powder River Basin (PRB) in southeastern Montana and northeastern
Wyoming contains massive coal deposits with biologically generated coal bed methane …

Hydrogeochemistry and coal-associated bacterial populations from a methanogenic coal bed

EP Barnhart, EP Weeks, EJP Jones, DJ Ritter… - International Journal of …, 2016 - Elsevier
Biogenic coalbed methane (CBM), a microbially-generated source of natural gas trapped
within coal beds, is an important energy resource in many countries. Specific bacterial …

Mineral microniches control the diversity of subsurface microbial populations

AA Jones, PC Bennett - Geomicrobiology Journal, 2014 - Taylor & Francis
Rocks and minerals surround the subsurface microbial habitat, yet mineralogy and rock
chemistry are rarely considered when characterizing microbial diversity and the microbial …