On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule …

JM Bolivar, B Nidetzky - Biochimica et Biophysica Acta (BBA)-Proteins and …, 2020 - Elsevier
The integration of enzymes with solid materials is important in many biotechnological
applications, including the use of immobilized enzymes for biocatalytic synthesis. The …

Integration and synergy in protein-nanomaterial hybrids for biosensing: Strategies and in-field detection applications

L Zhang, Y Ying, Y Li, Y Fu - Biosensors and Bioelectronics, 2020 - Elsevier
Biosensors contribute a lot to the reliable and sensitive detection in various fields, especially
emerging trends of in-field and real-time detection for point-of-care diagnosis, food safety …

Comparison of oriented and non-oriented antibody conjugation with AIE fluorescence microsphere for the immunochromatographic detection of enrofloxacin

L Hu, Z Chen, T Li, X Ye, Q Luo, W Lai - Food Chemistry, 2023 - Elsevier
Antibodies and labels were typically non-oriented conjugated in conventional
immunochromatographic assays (ICAs). In this work, a C-terminal cysteine-tagged …

Highly sensitive pregnancy test kit via oriented antibody conjugation on brush-type ligand-coated quantum beads

S Kim, Y Ahn, Y Bae, S Woo, J Park, H Kim… - Biosensors and …, 2022 - Elsevier
Lateral flow assays (LFA) enable development of portable and rapid diagnostic kits;
however, their capacity to detect low levels of disease markers remains poor. Here, we …

[HTML][HTML] LC-MS characterization of antibody-based therapeutics: recent highlights and future prospects

AC Robotham, JF Kelly - Approaches to the Purification, Analysis and …, 2020 - Elsevier
Antibody-based therapeutics constitute a major growth area in medicine today. However,
antibodies as drugs present significant analytical challenges as they are large, complex and …

Analytical methods of antibody surface coverage and orientation on bio-functionalized magnetic beads: application to immunocapture of TNF-α

E Laborie, V Le-Minh, TD Mai, M Ammar… - Analytical and …, 2021 - Springer
The use of magnetic beads bio-functionalized by antibodies (Ab) is constantly increasing
with a wide range of biomedical applications. However, despite an urgent need for current …

[HTML][HTML] Immuno-capture of biomolecules on functionalized magnetic beads: from characterization to fluorescent detection of a biomarker for Alzheimer's disease …

NVT Nguyen, C Smadja, M Taverna, TK Mai… - Talanta Open, 2023 - Elsevier
It is reported herein a new approach to study the orientation and density of mouse antibody
grafting on magnetic beads, serving for immunoassays and biosensors with fluorescent …

Organ-specific screening for protein damage using magnetic bead bioreactors and LC–MS/MS

D Jiang, M Shen, B Ahiadu, JF Rusling - Analytical chemistry, 2020 - ACS Publications
A new 96-well plate methodology for fast, enzyme-multiplexed screening for metabolite–
protein adducts was developed. Magnetic beads coated with metabolic enzymes were used …

Composition du Jury

A VARENNE, E LIPKA, F OUKACINE, B LE PIOUFLE… - theses.hal.science
The micro/nanometric magnetic particles, especially the beads constituted by Fe₃O₄ and
Fe₂O₃ are widely used today for diverse applications in the biomedical. In my thesis, I …