Evaluating metabolic stress and plasmid stability in plasmid DNA production by Escherichia coli
F Silva, JA Queiroz, FC Domingues - Biotechnology advances, 2012 - Elsevier
In the context of recombinant DNA technology, the development of feasible and high-
yielding plasmid DNA production processes has regained attention as more evidence for its …
yielding plasmid DNA production processes has regained attention as more evidence for its …
Advances in chromatographic supports for pharmaceutical‐grade plasmid DNA purification
 Sousa, F Sousa, JA Queiroz - Journal of separation science, 2012 - Wiley Online Library
Chromatographic technology is undoubtedly one of the most diverse and powerful
purification methods for downstream process applications. The diversity and quantity of …
purification methods for downstream process applications. The diversity and quantity of …
Enhancing E. coli Tolerance towards Oxidative Stress via Engineering Its Global Regulator cAMP Receptor Protein (CRP)
Oxidative damage to microbial hosts often occurs under stressful conditions during
bioprocessing. Classical strain engineering approaches are usually both time-consuming …
bioprocessing. Classical strain engineering approaches are usually both time-consuming …
Bacterial RNA extraction and purification from whole human blood using isotachophoresis
We demonstrate a novel assay for physicochemical extraction and isotachophoresis-based
purification of 16S rRNA from whole human blood infected with Pseudomonas putida. This …
purification of 16S rRNA from whole human blood infected with Pseudomonas putida. This …
[图书][B] Plasmid biopharmaceuticals: basics, applications, and manufacturing
DMF Prazeres - 2011 - books.google.com
The book addresses the basics, applications, and manufacturing of plasmid
biopharmaceuticals. The survey of the most relevant characteristics of plasmids provides the …
biopharmaceuticals. The survey of the most relevant characteristics of plasmids provides the …
Targeted gene therapy for the treatment of heart failure
K Rapti, AH Chaanine, RJ Hajjar - Canadian Journal of Cardiology, 2011 - Elsevier
Chronic heart failure is one of the leading causes of morbidity and mortality in Western
countries and is a major financial burden to the health care system. Pharmacologic …
countries and is a major financial burden to the health care system. Pharmacologic …
Development of a non-viral gene delivery vector based on the dynein light chain Rp3 and the TAT peptide
MTP Favaro, MAS de Toledo, RF Alves… - Journal of …, 2014 - Elsevier
Gene therapy and DNA vaccination trials are limited by the lack of gene delivery vectors that
combine efficiency and safety. Hence, the development of modular recombinant proteins …
combine efficiency and safety. Hence, the development of modular recombinant proteins …
Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery
The low efficiency of gene transfer is a recurrent problem in DNA vaccine development and
gene therapy studies using non-viral vectors such as plasmid DNA (pDNA). This is mainly …
gene therapy studies using non-viral vectors such as plasmid DNA (pDNA). This is mainly …
Enhanced biosynthesis of plasmid DNA from Escherichia coli VH33 using Box–Behnken design associated to aromatic amino acids pathway
Therapeutic applications of plasmid DNA have significantly advanced during the last years
and future demand for plasmid DNA requires the development of efficient bioprocesses …
and future demand for plasmid DNA requires the development of efficient bioprocesses …
Recombinant protein-based nanocarriers and their association with cationic liposomes: characterization and in vitro evaluation
RF Alves, MTP Favaro, TA Balbino… - Colloids and Surfaces A …, 2017 - Elsevier
A major bottleneck in the development of efficient protocols for gene therapy and DNA
vaccination is the low efficiency of gene transfer by non-viral vectors. This is mainly …
vaccination is the low efficiency of gene transfer by non-viral vectors. This is mainly …