Engineering stem cell self-organization to build better organoids
JA Brassard, MP Lutolf - Cell stem cell, 2019 - cell.com
Organoids form through self-organization processes in which initially homogeneous
populations of stem cells spontaneously break symmetry and undergo in-vivo-like pattern …
populations of stem cells spontaneously break symmetry and undergo in-vivo-like pattern …
Stem-cell-based embryo models for fundamental research and translation
Despite its importance, understanding the early phases of human development has been
limited by availability of human samples. The recent emergence of stem-cell-derived embryo …
limited by availability of human samples. The recent emergence of stem-cell-derived embryo …
Self-organization of stem cells into embryos: a window on early mammalian development
Embryonic development is orchestrated by robust and complex regulatory mechanisms
acting at different scales of organization. In vivo studies are particularly challenging for …
acting at different scales of organization. In vivo studies are particularly challenging for …
Dissecting the dynamics of signaling events in the BMP, WNT, and NODAL cascade during self-organized fate patterning in human gastruloids
During gastrulation, the pluripotent epiblast self-organizes into the 3 germ layers—
endoderm, mesoderm and ectoderm, which eventually form the entire embryo. Decades of …
endoderm, mesoderm and ectoderm, which eventually form the entire embryo. Decades of …
[HTML][HTML] Mouse gastrulation: Coordination of tissue patterning, specification and diversification of cell fate
ES Bardot, AK Hadjantonakis - Mechanisms of development, 2020 - Elsevier
During mouse embryonic development a mass of pluripotent epiblast tissue is transformed
during gastrulation to generate the three definitive germ layers: endoderm, mesoderm, and …
during gastrulation to generate the three definitive germ layers: endoderm, mesoderm, and …
Patterning principles of morphogen gradients
MF Simsek, EM Özbudak - Open Biology, 2022 - royalsocietypublishing.org
Metazoan embryos develop from a single cell into three-dimensional structured organisms
while groups of genetically identical cells attain specialized identities. Cells of the …
while groups of genetically identical cells attain specialized identities. Cells of the …
Engineering multiscale structural orders for high-fidelity embryoids and organoids
Embryoids and organoids hold great promise for human biology and medicine. Herein, we
discuss conceptual and technological frameworks useful for developing high-fidelity …
discuss conceptual and technological frameworks useful for developing high-fidelity …
The many bits of positional information
Half a century after Lewis Wolpert's seminal conceptual advance on how cellular fates
distribute in space, we provide a brief historical perspective on how the concept of positional …
distribute in space, we provide a brief historical perspective on how the concept of positional …
Efficient differentiation of human primordial germ cells through geometric control reveals a key role for Nodal signaling
Human primordial germ cells (hPGCs) form around the time of implantation and are the
precursors of eggs and sperm. Many aspects of hPGC specification remain poorly …
precursors of eggs and sperm. Many aspects of hPGC specification remain poorly …
Nodal is a short-range morphogen with activity that spreads through a relay mechanism in human gastruloids
Morphogens are signaling molecules that convey positional information and dictate cell
fates during development. Although ectopic expression in model organisms suggests that …
fates during development. Although ectopic expression in model organisms suggests that …