Genome editing for plant research and crop improvement
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a
profound impact on plant biology, and crop improvement. In this review, we summarize the …
profound impact on plant biology, and crop improvement. In this review, we summarize the …
Molecular mechanisms underlying host-induced gene silencing
H Zand Karimi, RW Innes - The Plant Cell, 2022 - academic.oup.com
Host-induced gene silencing (HIGS) refers to the silencing of genes in pathogens and pests
by expressing homologous double-stranded RNAs (dsRNA) or artificial microRNAs …
by expressing homologous double-stranded RNAs (dsRNA) or artificial microRNAs …
Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice
The CRISPR/Cas9 system is an efficient tool used for genome editing in a variety of
organisms. Despite several recent reports of successful targeted mutagenesis using the …
organisms. Despite several recent reports of successful targeted mutagenesis using the …
RNA silencing in plants: mechanisms, technologies and applications in horticultural crops
Q Guo, Q Liu, N A. Smith, G Liang… - Current genomics, 2016 - benthamdirect.com
Understanding the fundamental nature of a molecular process or a biological pathway is
often a catalyst for the development of new technologies in biology. Indeed, studies from late …
often a catalyst for the development of new technologies in biology. Indeed, studies from late …
DNA demethylases target promoter transposable elements to positively regulate stress responsive genes in Arabidopsis
TN Le, U Schumann, NA Smith, S Tiwari, PCK Au… - Genome biology, 2014 - Springer
Background DNA demethylases regulate DNA methylation levels in eukaryotes. Arabidopsis
encodes four DNA demethylases, DEMETER (DME), REPRESSOR OF SILENCING 1 …
encodes four DNA demethylases, DEMETER (DME), REPRESSOR OF SILENCING 1 …
CRISPR/Cas9-Mediated Targeted Mutagenesis in the Liverwort Marchantia polymorpha L.
SS Sugano, M Shirakawa, J Takagi… - Plant and Cell …, 2014 - academic.oup.com
Targeted genome modification technologies are key tools for functional genomics. The
clustered regularly interspaced short palindromic repeats (CRISPR)-associated …
clustered regularly interspaced short palindromic repeats (CRISPR)-associated …
Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression
L Long, DD Guo, W Gao, WW Yang, LP Hou, XN Ma… - Plant Methods, 2018 - Springer
Abstract Background When developing CRISPR/Cas9 systems for crops, it is crucial to
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …
invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes …
Engineering introns to express RNA guides for Cas9-and Cpf1-mediated multiplex genome editing
D Ding, K Chen, Y Chen, H Li, K Xie - Molecular plant, 2018 - cell.com
The clustered regularly interspaced short palindromic repeat (CRISPR) system has emerged
as the revolutionary platform for DNA targeting. This system uses a site-specific RNA guide …
as the revolutionary platform for DNA targeting. This system uses a site-specific RNA guide …
A stepwise pathway for biogenesis of 24‐nt secondary siRNAs and spreading of DNA methylation
We used a transgene system to study spreading of RNA‐directed DNA methylation (RdDM)
during transcriptional gene silencing in Arabidopsis thaliana. Forward and reverse genetics …
during transcriptional gene silencing in Arabidopsis thaliana. Forward and reverse genetics …
Dose-dependent RNAi-mediated geminivirus resistance in the tropical root crop cassava
H Vanderschuren, A Alder, P Zhang… - Plant molecular biology, 2009 - Springer
Cassava mosaic disease is a major constraint for cassava production in Africa, resulting in
significant economic losses. We have engineered transgenic cassava with resistance to …
significant economic losses. We have engineered transgenic cassava with resistance to …