Recombinant antibodies are highly specific detection probes in research, diagnostics, and
have emerged over the last two decades as the fastest growing class of therapeutic proteins …

Mammalian cells have developed dedicated molecular mechanisms to tightly control
expression levels of their genes where the specific transcriptomic signature across all genes …

Monoclonal antibody (mAb) therapeutics are an effective modality for the treatment of
infectious, autoimmune, and cancer-related diseases. However, the discovery, development …

Linking the heavy chain (HC) and light chain (LC) genes required for monoclonal antibodies
(mAb) production on a single cassette using 2A peptides allows control of LC and HC ratio …

Antibodies are indispensable tools for basic research as well as diagnostic and therapeutic
applications. Consequently, the development of alternative manufacturing strategies which …

The need for co-ordinate, high-level, and stable expression of multiple genes is essential for
the engineering of biosynthetic circuits and metabolic pathways. This work outlines the …

Immunoglobulin G (IgG), the most common class of commercial monoclonal antibodies
(mAbs), exists as multimers of two identical light chains (LC) and two identical heavy chains …

Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC),
and dihydrofolate reductase (DHFR) in one transcript were designed to compare internal …

Genes in the protein secretion pathway have been targeted to increase productivity of
monoclonal antibodies in Chinese hamster ovary cells. The results have been highly …

A set of mutated Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES)
elements with varying strengths is generated by mutating the translation initiation codons of …