Biochemistry of exosomes and their theranostic potential in human diseases

M Dwivedi, D Ghosh, A Saha, S Hasan, D Jindal… - Life Sciences, 2023 - Elsevier
Exosomes are classified as special extracellular vesicles in the eukaryotic system having
diameters ranging from 30 to 120 nm. These vesicles carry various endogenous molecules …

Engineering extracellular vesicles by three‐dimensional dynamic culture of human mesenchymal stem cells

X Yuan, L Sun, R Jeske, D Nkosi… - Journal of …, 2022 - Wiley Online Library
Human mesenchymal stem cell (hMSC) derived extracellular vesicles (EVs) have shown
therapeutic potential in recent studies. However, the corresponding therapeutic components …

Upscaling human mesenchymal stromal cell production in a novel vertical-wheel bioreactor enhances extracellular vesicle secretion and cargo profile

R Jeske, C Liu, L Duke, MLC Castro, L Muok, P Arthur… - Bioactive Materials, 2023 - Elsevier
Human mesenchymal stromal cells (hMSCs) are mechanically sensitive undergoing
phenotypic alterations when subjected to shear stress, cell aggregation, and substrate …

Tetraspanin CD63 bridges autophagic and endosomal processes to regulate exosomal secretion and intracellular signaling of Epstein-Barr virus LMP1

SN Hurwitz, MR Cheerathodi, D Nkosi, SB York… - Journal of …, 2018 - Am Soc Microbiol
The tetraspanin protein CD63 has been recently described as a key factor in extracellular
vesicle (EV) production and endosomal cargo sorting. In the context of Epstein-Barr virus …

Application of engineered extracellular vesicles for targeted tumor therapy

F Zhang, J Guo, Z Zhang, M Duan, G Wang… - Journal of biomedical …, 2022 - Springer
All cells, including prokaryotes and eukaryotes, could release extracellular vesicles (EVs).
EVs contain many cellular components, including RNA, and surface proteins, and are …

Surface engineering of HEK293 cell-derived extracellular vesicles for improved pharmacokinetic profile and targeted delivery of IL-12 for the treatment of …

J Zhang, H Song, Y Dong, G Li, J Li, Q Cai… - International journal …, 2023 - Taylor & Francis
Background Extracellular vesicles (EVs) are considered a promising drug delivery platform.
Naïve EVs face numerous issues that limit their applications, such as fast clearance, hepatic …

CCR2 cooperativity promotes hematopoietic stem cell homing to the bone marrow

SN Hurwitz, DR Kobulsky, SK Jung, JJ Chia… - Science …, 2024 - science.org
Cross-talk between hematopoietic stem and progenitor cells (HSPCs) and bone marrow
(BM) cells is critical for homing and sustained engraftment after transplantation. In particular …

Extracellular vesicle integrins distinguish unique cancers

SN Hurwitz, DG Meckes Jr - Proteomes, 2019 - mdpi.com
The proteomic profile of extracellular vesicles (EVs) has been of increasing interest,
particularly in understanding cancer growth, drug resistance, and metastatic behavior …

Extraction of extracellular vesicles from whole tissue

SN Hurwitz, JM Olcese… - Journal of visualized …, 2019 - pmc.ncbi.nlm.nih.gov
Circulating and interstitial small membrane-bound extracellular vesicles (EVs) represent
promising targets for the development of novel diagnostic or prognostic biomarker assays …

An optimized method for enrichment of whole brain-derived extracellular vesicles reveals insight into neurodegenerative processes in a mouse model of Alzheimer's …

SN Hurwitz, L Sun, KY Cole, CR Ford III… - Journal of neuroscience …, 2018 - Elsevier
Background Alzheimer's disease (AD) is the major cause of dementia that has increased
dramatically in prevalence over the past several decades. Yet many questions still surround …