Structural biology of CRISPR–Cas immunity and genome editing enzymes
CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …
a wide range of genome editing and biotechnological applications. In this Review, we …
Controlling and enhancing CRISPR systems
Many bacterial and archaeal organisms use clustered regularly interspaced short
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
palindromic repeats–CRISPR associated (CRISPR–Cas) systems to defend themselves …
Bacteriophages suppress CRISPR–Cas immunity using RNA-based anti-CRISPRs
Many bacteria use CRISPR–Cas systems to combat mobile genetic elements, such as
bacteriophages and plasmids. In turn, these invasive elements have evolved anti-CRISPR …
bacteriophages and plasmids. In turn, these invasive elements have evolved anti-CRISPR …
Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo
Adeno-associated virus (AAV) vectors are important delivery platforms for therapeutic
genome editing but are severely constrained by cargo limits. Simultaneous delivery of …
genome editing but are severely constrained by cargo limits. Simultaneous delivery of …
Inhibitors of bacterial immune systems: discovery, mechanisms and applications
D Mayo-Muñoz, R Pinilla-Redondo… - Nature Reviews …, 2024 - nature.com
To contend with the diversity and ubiquity of bacteriophages and other mobile genetic
elements, bacteria have developed an arsenal of immune defence mechanisms. Bacterial …
elements, bacteria have developed an arsenal of immune defence mechanisms. Bacterial …
CRISPR modeling and correction of cardiovascular disease
Cardiovascular disease remains the leading cause of morbidity and mortality in the
developed world. In recent decades, extraordinary effort has been devoted to defining the …
developed world. In recent decades, extraordinary effort has been devoted to defining the …
Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins
CRISPR loci and Cas proteins provide adaptive immunity in prokaryotes against invading
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …
CRISPR-Cas-based antimicrobials: design, challenges, and bacterial mechanisms of resistance
A Mayorga-Ramos, J Zúñiga-Miranda… - ACS Infectious …, 2023 - ACS Publications
The emergence of antibiotic-resistant bacterial strains is a source of public health concern
across the globe. As the discovery of new conventional antibiotics has stalled significantly …
across the globe. As the discovery of new conventional antibiotics has stalled significantly …
Drivers and consequences of bacteriophage host range
D Holtappels, P Alfenas-Zerbini… - FEMS Microbiology …, 2023 - academic.oup.com
Bacteriophages are obligate parasites of bacteria characterized by the breadth of hosts that
they can infect. This “host range” depends on the genotypes and morphologies of the phage …
they can infect. This “host range” depends on the genotypes and morphologies of the phage …
Discovery of multiple anti-CRISPRs highlights anti-defense gene clustering in mobile genetic elements
Many prokaryotes employ CRISPR–Cas systems to combat invading mobile genetic
elements (MGEs). In response, some MGEs have developed strategies to bypass immunity …
elements (MGEs). In response, some MGEs have developed strategies to bypass immunity …