Cornerstones of CRISPR–Cas in drug discovery and therapy
C Fellmann, BG Gowen, PC Lin, JA Doudna… - Nature reviews Drug …, 2017 - nature.com
The recent development of CRISPR–Cas systems as easily accessible and programmable
tools for genome editing and regulation is spurring a revolution in biology. Paired with the …
tools for genome editing and regulation is spurring a revolution in biology. Paired with the …
[HTML][HTML] Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases
The development of customizable sequence-specific nucleases such as TALENs, ZFNs and
the powerful CRISPR/Cas9 system has revolutionized the field of genome editing. The …
the powerful CRISPR/Cas9 system has revolutionized the field of genome editing. The …
Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors
Studying the role of essential proteins is dependent upon a method for rapid inactivation, in
order to study the immediate phenotypic consequences. Auxin-inducible degron (AID) …
order to study the immediate phenotypic consequences. Auxin-inducible degron (AID) …
The auxin-inducible degradation (AID) system enables versatile conditional protein depletion in C. elegans
Experimental manipulation of protein abundance in living cells or organisms is an essential
strategy for investigation of biological regulatory mechanisms. Whereas powerful techniques …
strategy for investigation of biological regulatory mechanisms. Whereas powerful techniques …
High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes
Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease
Cas9 has become a popular method for genome editing in several organisms. Most HDR …
Cas9 has become a popular method for genome editing in several organisms. Most HDR …
Streamlined genome engineering with a self-excising drug selection cassette
A central goal in the development of genome engineering technology is to reduce the time
and labor required to produce custom genome modifications. Here we describe a new …
and labor required to produce custom genome modifications. Here we describe a new …
Melting dsDNA Donor Molecules Greatly Improves Precision Genome Editing in Caenorhabditis elegans
KS Ghanta, CC Mello - Genetics, 2020 - academic.oup.com
Melting and fast cooling double stranded DNA donor molecules prior to injection
dramatically increases the frequency of homology-directed repair for edits such as insertions …
dramatically increases the frequency of homology-directed repair for edits such as insertions …
CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering
DJ Dickinson, B Goldstein - Genetics, 2016 - academic.oup.com
The advent of genome editing techniques based on the clustered regularly interspersed
short palindromic repeats (CRISPR)–Cas9 system has revolutionized research in the …
short palindromic repeats (CRISPR)–Cas9 system has revolutionized research in the …
An expanded auxin-inducible degron toolkit for Caenorhabditis elegans
GE Ashley, T Duong, MT Levenson, MAQ Martinez… - Genetics, 2021 - academic.oup.com
The auxin-inducible degron (AID) system has emerged as a powerful tool to conditionally
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …
deplete proteins in a range of organisms and cell types. Here, we describe a toolkit to …
[HTML][HTML] Improved genome editing efficiency and flexibility using modified oligonucleotides with TALEN and CRISPR-Cas9 nucleases
JB Renaud, C Boix, M Charpentier, A De Cian… - Cell reports, 2016 - cell.com
Genome editing has now been reported in many systems using TALEN and CRISPR-Cas9
nucleases. Precise mutations can be introduced during homology-directed repair with donor …
nucleases. Precise mutations can be introduced during homology-directed repair with donor …