Comparative analysis of double-stranded RNA degradation and processing in insects

IK Singh, S Singh, K Mogilicherla, JN Shukla… - Scientific reports, 2017 - nature.com
RNA interference (RNAi) based methods are being developed for pest management. A few
products for control of coleopteran pests are expected to be commercialized soon. However …

Humanizing glycosylation pathways in eukaryotic expression systems

AH Khan, H Bayat, M Rajabibazl, S Sabri… - World Journal of …, 2017 - Springer
Glycosylation represents the most widespread posttranslational modifications, found in a
broad spectrum of natural and therapeutic recombinant proteins. It highly affects bioactivity …

Down-regulation of cytokinin oxidase 2 expression increases tiller number and improves rice yield

SY Yeh, HW Chen, CY Ng, CY Lin, TH Tseng, WH Li… - Rice, 2015 - Springer
Background Cytokinins are plant-specific hormones that affect plant growth and
development. The endogenous level of cytokinins in plant cells is regulated in part by …

An overview and history of glyco-engineering in insect expression systems

C Geisler, H Mabashi-Asazuma, DL Jarvis - Glyco-Engineering: Methods …, 2015 - Springer
Insect systems, including the baculovirus-insect cell and Drosophila S2 cell systems are
widely used as recombinant protein production platforms. Historically, however, no insect …

CRISPR-Cas9 vectors for genome editing and host engineering in the baculovirus–insect cell system

H Mabashi-Asazuma, DL Jarvis - Proceedings of the …, 2017 - National Acad Sciences
The baculovirus–insect cell system (BICS) has been widely used to produce many different
recombinant proteins for basic research and is being used to produce several biologics …

Functional analysis of the RNAi response in ovary-derived silkmoth Bm5 cells

A Kolliopoulou, L Swevers - Insect biochemistry and molecular biology, 2013 - Elsevier
Experiments of dsRNA-mediated gene silencing in lepidopteran insects in vivo are
characterized by high variability although lepidopteran cell cultures have shown an efficient …

Glycobiotechnology of the insect cell-baculovirus expression system technology

LA Palomares, IK Srivastava, OT Ramírez… - Advances in …, 2018 - Springer
The insect cell-baculovirus expression system technology (BEST) has a prominent role in
producing recombinant proteins to be used as research and diagnostic reagents and …

Modifying an Insect Cell N-Glycan Processing Pathway Using CRISPR-Cas Technology

H Mabashi-Asazuma, CW Kuo, KH Khoo… - ACS Chemical …, 2015 - ACS Publications
Fused lobes (FDL) is an enzyme that simultaneously catalyzes a key trimming reaction and
antagonizes elongation reactions in the insect N-glycan processing pathway. Accordingly …

Establishment of an integrated CRISPR/Cas9 plasmid system for simple and efficient genome editing in medaka in vitro and in vivo

Z Zhang, J Wang, J Li, X Liu, L Liu, C Zhao, W Tao… - Biology, 2023 - mdpi.com
Simple Summary The application of CRISPR/Cas9 genome editing in fish is limited to date.
An easy-to-use, economical and effective genome editing system is greatly needed. In this …

RNA interference technology to improve the baculovirus-insect cell expression system

C Chavez-Pena, AA Kamen - Biotechnology advances, 2018 - Elsevier
The baculovirus expression vector system (BEVS) is a popular manufacturing platform for
the production of recombinant proteins, antiviral vaccines, gene therapy vectors, and …