Mechanism of membrane-curvature generation by ER-tubule shaping proteins

N Wang, LD Clark, Y Gao, MM Kozlov… - Nature …, 2021 - nature.com
The endoplasmic reticulum (ER) network consists of tubules with high membrane curvature
in cross-section, generated by the reticulons and REEPs. These proteins have two pairs of …

The perinuclear ER scales nuclear size independently of cell size in early embryos

RN Mukherjee, J Sallé, S Dmitrieff, KM Nelson… - Developmental cell, 2020 - cell.com
Nuclear size plays pivotal roles in gene expression, embryo development, and disease. A
central hypothesis in organisms ranging from yeast to vertebrates is that nuclear size scales …

Peroxisome protein import recapitulated in Xenopus egg extracts

FB Romano, NB Blok, TA Rapoport - Journal of Cell Biology, 2019 - rupress.org
Peroxisomes import their luminal proteins from the cytosol. Most substrates contain a C-
terminal Ser-Lys-Leu (SKL) sequence that is recognized by the receptor Pex5. Pex5 binds to …

The use of cell-free Xenopus extracts to investigate cytoplasmic events

R Gibeaux, R Heald - Cold Spring Harbor Protocols, 2019 - cshprotocols.cshlp.org
Experiments using cytoplasmic extracts prepared from Xenopus eggs have made important
contributions to our understanding of the cell cycle, the cytoskeleton, and cytoplasmic …

[HTML][HTML] Xenopus cell-free extracts and their applications in cell biology study

J Liu, C Zhang - Biophysics Reports, 2023 - ncbi.nlm.nih.gov
Xenopus has proven to be a remarkably versatile model organism in the realm of biological
research for numerous years, owing to its straightforward maintenance in laboratory settings …

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