Monoliths have consolidated their position in bioseparation. More than 200 different
applications have been reported in the past two decades and their advantages compared to …

Columns for liquid chromatography produce ever narrower peaks and analysts continue to
push to smaller column diameters to save solvents and have better MS responses. The …

The effect of porous structures of 2-mm thick diethylamine functionalized monolithic
polymethacrylate discs on their chromatographic behavior in ion-exchange mode has been …

Chromatography is the workhorse of biopharmaceutical downstream processing because it
can selectively enrich a target product while removing impurities from complex feed streams …

Adsorptive membranes were investigated for the downstream processing of plasmid DNA by
quantifying both separation efficiencies and adsorption uptake with the anion-exchange …

In preparative chromatography, a uniform flow distribution through the chromatography
device is essential to get an effective ligand utilization and a breakthrough curve that is as …

The flow reversal (FR) technique consists of reversing the flow direction along a
chromatographic column. It is used to reveal the origin (such as poor column packing, active …

New therapeutics that are being developed rely more and more on large and complex
biomacromolecules like proteins, DNA, and viral particles. Manufacturing processes are …

Molecularly imprinted polymer membranes for a model compound, atrazine, were prepared
in situ from a monomer mixture containing methacrylic acid, tri (ethylene glycol) …

It becomes increasingly clear that flow non-uniformity frequently limits the performance of
chromatographic columns, and that there are at least two major causes for this: non-uniform …