Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

M Ahmad, M Hirz, H Pichler, H Schwab - Applied microbiology and …, 2014 - Springer
Pichia pastoris is an established protein expression host mainly applied for the production of
biopharmaceuticals and industrial enzymes. This methylotrophic yeast is a distinguished …

Heterologous protein expression in the methylotrophic yeast Pichia pastoris

JL Cereghino, JM Cregg - FEMS microbiology reviews, 2000 - academic.oup.com
During the past 15 years, the methylotrophic yeast Pichia pastoris has developed into a
highly successful system for the production of a variety of heterologous proteins. The …

[HTML][HTML] Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

A Weninger, AM Hatzl, C Schmid, T Vogl… - Journal of …, 2016 - Elsevier
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is one of the most commonly
used expression systems for heterologous protein production. However the recombination …

Heterologous Protein Expression in Pichia pastoris: Latest Research Progress and Applications

V Juturu, JC Wu - ChemBioChem, 2018 - Wiley Online Library
Pichia pastoris is a well‐known platform strain for heterologous protein expression. Over the
past five years, different strategies to improve the efficiency of recombinant protein …

Recombinant protein expression in Pichia pastoris

JM Cregg, JL Cereghino, J Shi, DR Higgins - Molecular biotechnology, 2000 - Springer
The methylotrophic yeast Pichia pastoris is now one of the standard tools used in molecular
biology for the generation of recombinant protein. P. pastoris has demonstrated its most …

Production of recombinant proteins by yeast cells

E Çelik, P Çalık - Biotechnology advances, 2012 - Elsevier
Yeasts are widely used in production of recombinant proteins of medical or industrial
interest. For each individual product, the most suitable expression system has to be …

Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production

R Daly, MTW Hearn - Journal of Molecular Recognition: An …, 2005 - Wiley Online Library
The use of the methylotrophic yeast, Pichia pastoris, as a cellular host for the expression of
recombinant proteins has become increasing popular in recent times. P. pastoris is easier to …

Recombinant protein production in yeasts

D Mattanovich, P Branduardi, L Dato, B Gasser… - Recombinant gene …, 2012 - Springer
Recombinant protein production is a multibillion-dollar market. The development of a new
product begins with the choice of a production host. While one single perfect host for every …

Regulation of Pichia pastoris promoters and its consequences for protein production

T Vogl, A Glieder - New biotechnology, 2013 - Elsevier
The methylotrophic yeast Pichia pastoris is a widely used host for heterologous protein
production. Along with favorable properties such as growth to high cell density and high …

First cloning and characterization of two functional aquaporin genes from an arbuscular mycorrhizal fungus Glomus intraradices

T Li, YJ Hu, ZP Hao, H Li, YS Wang… - New Phytologist, 2013 - Wiley Online Library
Arbuscular mycorrhizal (AM) symbiosis is known to stimulate plant drought tolerance.
However, the molecular basis for the direct involvement of AM fungi (AMF) in plant water …