We present a protocol for the generation of a gene-deletion allelic-exchange plasmid and its
recovery in Escherichia coli for the purpose of constructing an in-frame gene deletion in …

Gene deletions can be generated in Staphylococcus aureus using recombineering in
combination with a CRISPR–Cas9 counterselection approach. The method involves first …

Here we describe an allelic-exchange procedure for the construction of an unmarked gene
deletion in the bacterium Staphylococcus aureus. As a practical example, we outline the …

This protocol continues a series of methods for the construction of an in-frame gene deletion
in Staphylococcus aureus strain RN4220. To this end, we describe in this protocol an allelic …

Methods for gene disruption are essential for functional genomics, and there are multiple
approaches for altering gene function in bacteria. One of these methods involves introducing …