Gene deletions can be generated in Staphylococcus aureus using recombineering in combination with a CRISPR–Cas9 counterselection approach. The method involves first …
MS Zeden, CF Schuster… - Cold Spring Harbor …, 2023 - cshprotocols.cshlp.org
Here we describe an allelic-exchange procedure for the construction of an unmarked gene deletion in the bacterium Staphylococcus aureus. As a practical example, we outline the …
MS Zeden, CF Schuster… - Cold Spring Harbor …, 2023 - cshprotocols.cshlp.org
This protocol continues a series of methods for the construction of an in-frame gene deletion in Staphylococcus aureus strain RN4220. To this end, we describe in this protocol an allelic …
A Gründling, Q Ji - Cold Spring Harbor Protocols, 2024 - cshprotocols.cshlp.org
Methods for gene disruption are essential for functional genomics, and there are multiple approaches for altering gene function in bacteria. One of these methods involves introducing …