▪ Abstract Structural models of site-specific recombinases from the lambda integrase family
of enzymes have in the last four years provided an important new perspective on the three …

The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert
DNA cassettes into eukaryotic chromosomes is widespread (1–9). Indeed, a PubMed search …

The resolution and dimensionality with which biologists can characterize cell types have
expanded dramatically in recent years, and intersectional consideration of such features (eg …

The use of synthetic biological systems in research, healthcare, and manufacturing often
requires autonomous history-dependent behavior and therefore some form of engineered …

Functional redundancies, compensatory mechanisms, and lethal phenotypes often prevent
the full analysis of gene functions through generation of germline null mutations in the …

Cre recombinase from bacteriophage P1 recognizes a 34-bp recombination site, loxP, with
exquisite sequence specificity and catalyzes the site-specific insertion, excision, or …

Cre recombinase catalyzes site-specific recombination between two 34-bp loxP sites in a
variety of DNA substrates. At the start of the recombination pathway, the loxP sites are each …

G-protein receptor kinase 2 (GRK2) is 1 of 7 mammalian GRKs that phosphorylate ligand-
bound 7-transmembrane receptors, causing receptor uncoupling from G proteins and …

In the postgenomic era the mouse will be central to the challenge of ascribing a function to
the 40,000 or so genes that constitute our genome. In this review, we summarize some of the …

The probability that two sites on a linear DNA molecule will contact each other by looping
depends on DNA flexibility. Although the flexibility of naked DNA in vitro is well …