Mass spectrometry methods for measuring protein stability

DD Vallejo, C Rojas Ramírez, KF Parson, Y Han… - Chemical …, 2022 - ACS Publications
Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

From natural bone grafts to tissue engineering therapeutics: brainstorming on pharmaceutical formulative requirements and challenges

B Baroli - Journal of pharmaceutical sciences, 2009 - Elsevier
Tissue engineering is an emerging multidisciplinary field of investigation focused on the
regeneration of diseased or injured tissues through the delivery of appropriate molecular …

Structural stability from solution to the gas phase: native solution structure of ubiquitin survives analysis in a solvent-free ion mobility–mass spectrometry environment

T Wyttenbach, MT Bowers - The journal of physical chemistry B, 2011 - ACS Publications
The conformations of desolvated ubiquitin ions, lifted into the gas phase by electrospray
ionization (ESI), were characterized by ion mobility spectrometry (IMS) and compared to the …

Probing protein ligand interactions by automated hydrogen/deuterium exchange mass spectrometry

MJ Chalmers, SA Busby, BD Pascal, Y He… - Analytical …, 2006 - ACS Publications
Amide hydrogen/deuterium exchange is a powerful biophysical technique for probing
changes in protein dynamics induced by ligand interaction. The inherent low throughput of …

Promiscuous aggregate-based inhibitors promote enzyme unfolding

KED Coan, DA Maltby, AL Burlingame… - Journal of medicinal …, 2009 - ACS Publications
One of the leading sources of false positives in early drug discovery is the formation of
organic small molecule aggregates, which inhibit enzymes nonspecifically at micromolar …

The application of terahertz spectroscopy to protein detection: a review

L Xie, Y Yao, Y Ying - Applied Spectroscopy Reviews, 2014 - Taylor & Francis
Terahertz (THz) technology is a recent promising technology that plays an important role in
various areas, including national defense, military, materials, chemistry, medicine …

Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi

G D'Angelo, T Uemura, CC Chuang, E Polishchuk… - Nature, 2013 - nature.com
Newly synthesized proteins and lipids are transported across the Golgi complex via different
mechanisms whose respective roles are not completely clear. We previously identified a non …

Protein structure and dynamics studied by mass spectrometry: H/D exchange, hydroxyl radical labeling, and related approaches

L Konermann, X Tong, Y Pan - Journal of Mass Spectrometry, 2008 - Wiley Online Library
Mass spectrometry (MS) plays a central role in studies on protein structure and dynamics.
This review highlights some of the recent developments in this area, with focus on …

Intramolecular migration of amide hydrogens in protonated peptides upon collisional activation

TJD Jørgensen, H Gårdsvoll, M Ploug… - Journal of the …, 2005 - ACS Publications
Presently different opinions exist as to the degree of scrambling of amide hydrogens in
gaseous protonated peptides and proteins upon collisional activation in tandem mass …

Arsenic binding to human metallothionein

TT Ngu, MJ Stillman - Journal of the American Chemical Society, 2006 - ACS Publications
The number of reported cases of chronic arsenic poisoning is on the rise throughout the
world, making the study of the long-term effects of arsenic critical. As3+ binds readily to …