Aberrations in telomere biology are among the earliest events in prostate cancer
tumorigenesis and continue during tumour progression. Substantial telomere shortening …

290 MURRAY & SZOSTAK was present in the parental cell. Chromosome segregation is an
extremely accurate process: Chromosome loss in mitosis in yeast occurs once in every 105 …

A series of yeast shuttle vectors and host strains has been created to allow more efficient
manipulation of DNA in Saccharomyces cerevisiae. Transplacement vectors were …

A set of four yeast shuttle vectors that incorporate sequences from the Saccharomyces
cerevisiae 2μ endogenous plasmid has been constructed. These yeast episomal plasmid …

DNA was introduced into the germ line of the nematode Caenorhabditis elegans by
microinjection. Approximately 10% of the injected worms gave rise to transformed progeny …

We have isolated yeast mutants that are defective in the maintenance of circular
minichromosomes. The minichromosomes are mitotically stable plasmids, each of which …

Fifty-five-kilobase long artificial chromosomes containing cloned genes, replicators,
centromeres and telomeres have been constructed in yeast. These molecules have many of …

Saccharomyces teiomeres consist of-300 bp of C,. & TGlms DNA. Nondenaturing Southern
hybridization, capable of detecting-60 to-300 bases of TGl. a DNA, revealed that yeast …

The protein encoded by the RAP7 gene of S. cerevisiae binds in vitro to a consensus
sequence occurring at a number of sites in the yeast genome, including the repeated …

We have devised a genetic selection for mutant yeast cells that fail to translocate secretory
protein precursors into the lumen of the endoplasmic reticulum (ER). Mutant cells are …