Molecular oxygen, O2, has long provided a cornerstone for studies in chemistry, physics,
and biology. Although the triplet ground state, O2 (X3Σg–), has garnered much attention, the …

Fluorescence-based single-molecule optical detection techniques are widely chosen over
other methods, owing to the ease of background screening and better signal-to-noise …

Plasmonic enhancement of two-photon-excited fluorescence is not only of fundamental
interest but also appealing for many bioimaging and photonic applications. The high peak …

We demonstrate two-photon-excited single-molecule fluorescence enhancement by single
end-to-end self-assembled gold nanorod dimers. We employed biotinylated streptavidin as …

Optical detection of individual nanometer-sized analytes, virus particles, and protein
molecules holds great promise for understanding and control of biological samples and …

Porphyrins are typically weak emitters, which presents challenges to their optical detection
by single-molecule fluorescence microscopy. In this contribution, we explore the …

Measuring the orientation dynamics of nanoparticles and nonfluorescent molecules in real
time with optical methods is still a challenge in nanoscience and biochemistry. Here, we …

Biomolecules exhibit dynamics on widely varying timescales, which are critical to their
functioning in living systems. Particularly sub-millisecond dynamics, such as low-affinity …

SUMMARY A Golden Key to Microseconds: Plasmon-Enhanced Single-Molecule
Fluorescence for Ultrafast Biomolecular Dynamics Biomolecules such as nucleic acids and …

Single-molecule measurements are now almost routinely used to study biological systems
and processes. The scope of this special topic emphasizes the physics side of single …