High throughput sequencing for plant virus detection and discovery

DEV Villamor, T Ho, M Al Rwahnih, RR Martin… - …, 2019 - Am Phytopath Society
Over the last decade, virologists have discovered an unprecedented number of viruses
using high throughput sequencing (HTS), which led to the advancement of our knowledge …

VirusDetect: An automated pipeline for efficient virus discovery using deep sequencing of small RNAs

Y Zheng, S Gao, C Padmanabhan, R Li, M Galvez… - Virology, 2017 - Elsevier
Accurate detection of viruses in plants and animals is critical for agriculture production and
human health. Deep sequencing and assembly of virus-derived small interfering RNAs has …

Exogenously applied dsRNA molecules deriving from the Zucchini yellow mosaic virus (ZYMV) genome move systemically and protect cucurbits against ZYMV

A Kaldis, M Berbati, O Melita, C Reppa… - Molecular plant …, 2018 - Wiley Online Library
Zucchini yellow mosaic virus (ZYMV) causes serious damage in a large number of cucurbits,
and control measures are necessary. Transgenic cucurbits expressing parts of the ZYMV …

Towards the validation of high-throughput sequencing (HTS) for routine plant virus diagnostics: measurement of variation linked to HTS detection of citrus viruses and …

R Bester, G Cook, JHJ Breytenbach, C Steyn… - Virology Journal, 2021 - Springer
Background High-throughput sequencing (HTS) has been applied successfully for virus and
viroid discovery in many agricultural crops leading to the current drive to apply this …

De Novo Reconstruction of Consensus Master Genomes of Plant RNA and DNA Viruses from siRNAs

J Seguin, R Rajeswaran, N Malpica-Lopez, RR Martin… - PloS one, 2014 - journals.plos.org
Virus-infected plants accumulate abundant, 21–24 nucleotide viral siRNAs which are
generated by the evolutionary conserved RNA interference (RNAi) machinery that regulates …

The induction of an effective dsRNA-mediated resistance against tomato spotted wilt virus by exogenous application of double-stranded RNA largely depends on the …

S Tabein, M Jansen, E Noris, AM Vaira… - Frontiers in Plant …, 2020 - frontiersin.org
Tomato spotted wilt virus (TSWV) is a devastating plant pathogen, causing huge crop losses
worldwide. Unfortunately, due to its wide host range and emergence of resistance breaking …

Field trial and molecular characterization of RNAi-transgenic tomato plants that exhibit resistance to tomato yellow leaf curl geminivirus

A Fuentes, N Carlos, Y Ruiz, D Callard… - Molecular Plant …, 2016 - Am Phytopath Society
RNA interference (RNAi) is a widely used approach to generate virus-resistant transgenic
crops. However, issues of agricultural importance like the long-term durability of RNAi …

Atypical molecular features of RNA silencing against the phloem-restricted polerovirus TuYV

M Clavel, E Lechner, M Incarbone… - Nucleic Acids …, 2021 - academic.oup.com
In plants and some animal lineages, RNA silencing is an efficient and adaptable defense
mechanism against viruses. To counter it, viruses encode suppressor proteins that interfere …

Deep sequencing of mycovirus‐derived small RNAs from Botrytis species

L Donaire, MA Ayllón - Molecular Plant Pathology, 2017 - Wiley Online Library
RNA silencing is an ancient regulatory mechanism operating in all eukaryotic cells. In fungi,
it was first discovered in Neurospora crassa, although its potential as a defence mechanism …

An internet-based bioinformatics toolkit for plant biosecurity diagnosis and surveillance of viruses and viroids

RA Barrero, KR Napier, J Cunnington, L Liefting… - BMC …, 2017 - Springer
Background Detection and preventing entry of exotic viruses and viroids at the border is
critical for protecting plant industries trade worldwide. Existing post entry quarantine …