Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides

L Cabral, GF Persinoti, DAA Paixão, MP Martins… - Nature …, 2022 - nature.com
The largest living rodent, capybara, can efficiently depolymerize and utilize lignocellulosic
biomass through microbial symbiotic mechanisms yet elusive. Herein, we elucidate the …

Carbohydrate binding modules: Compact yet potent accessories in the specific substrate binding and performance evolution of carbohydrate-active enzymes

Y You, H Kong, C Li, Z Gu, X Ban, Z Li - Biotechnology Advances, 2024 - Elsevier
Carbohydrate binding modules (CBMs) are independent non-catalytic domains widely found
in carbohydrate-active enzymes (CAZymes), and they play an essential role in the substrate …

A novel class of xylanases specifically degrade marine red algal β1, 3/1, 4-mixed-linkage xylan

F Zhao, CM Yu, HN Sun, LS Zhao, HT Ding… - Journal of Biological …, 2023 - ASBMB
Xylans are polysaccharides composed of xylose and include β1, 4-xylan, β1, 3-xylan, and
β1, 3/1, 4-mixed-linkage xylan (MLX). MLX is widely present in marine red algae and …

A functionally augmented carbohydrate utilization locus from herbivore gut microbiota fueled by dietary β-glucans

F Mandelli, MP Martins, M Chinaglia, EA Lima… - npj Biofilms and …, 2024 - nature.com
Gut microbiota members from the Bacteroidota phylum play a pivotal role in mammalian
health and metabolism. They thrive in this diverse ecosystem due to their notable ability to …

Characterization of a novel GH26 β-mannanase from Paenibacillus polymyxa and its application in the production of mannooligosaccharides

J Gao, H Zheng, X Wang, Y Li - Enzyme and Microbial Technology, 2023 - Elsevier
A novel glycoside hydrolase family 26 β-mannanase gene ppman26a was cloned from
Paenibacillus polymyxa KF-1. The full-length enzyme PpMan26A and its truncated products …

Identification and biochemical characterization of a novel GH113 β-mannanase from acid mine drainage metagenome

J Peng, W Liu, S Tang, S Zou, Y Zhu, H Cheng… - Biochemical …, 2023 - Elsevier
Acid mine drainage (AMD) is a highly acidic and heavy metal-bearing environment with
marginal availability of nutrients. Indeed, recycling the scarce organic matter in such …

Reshaping the binding channel of a novel GH113 family β-mannanase from Paenibacillus cineris (PcMan113) for enhanced activity

D Sun, C Li, P Cui, J Zhang, Y Zhou, M Wu, X Li… - Bioresources and …, 2022 - Springer
Endo-β-mannanases are important enzymes for degrading lignocellulosic biomass to
generate mannan, which has significant health effects as a prebiotic that promotes the …

Unique active-site and subsite features in the arabinogalactan-degrading GH43 exo-β-1, 3-galactanase from Phanerochaete chrysosporium

K Matsuyama, N Kishine, Z Fujimoto… - Journal of Biological …, 2020 - ASBMB
Arabinogalactan proteins (AGPs) are plant proteoglycans with functions in growth and
development. However, these functions are largely unexplored, mainly because of the …

Application fields, positions, and bioinformatic mining of non-active sites: a mini-review

X Wang, Q Ma, J Shen, B Wang, X Gao… - Frontiers in Chemistry, 2021 - frontiersin.org
Active sites of enzymes play a vital role in catalysis, and researchhas been focused on the
interactions between active sites and substrates to understand the biocatalytic process …

Gut microbiome of capybara, the Amazon master of the grasses, harbors unprecedented enzymatic strategies for plant glycans breakdown

L Cabral, GF Persinoti, DAA Paixao, MP Martins… - 2021 - researchsquare.com
Background: Plant biomass is a promising feedstock to replace fossil-based products
including fuels, chemicals and materials. However, the high resistance of plant biomass to …