Horizontal gene transfer (HGT) is one of the most important evolutionary forces within
microbial populations. Although evidence for beneficial fitness effects of HGT is …

Transformation-associated recombination (TAR) cloning represents a unique tool for
isolation and manipulation of large DNA molecules. The technique exploits a high level of …

DNA digital storage provides an alternative for information storage with high density and
long-term stability. Here, we report the de novo design and synthesis of an artificial …

Conjugation of episomal plasmids from bacteria to diatoms advances diatom genetic
manipulation by simplifying transgene delivery and providing a stable and consistent gene …

There is remarkable variation in the rate at which genetic incompatibilities in molecular
interactions accumulate. In some cases, minor changes—even single-nucleotide …

Genome transplantation (GT) allows the installation of purified chromosomes into recipient
cells, causing the resulting organisms to adopt the genotype and the phenotype conferred …

In vitro recombination methods have enabled one-step construction of large DNA
sequences from multiple parts. Although synthetic biological circuits can in principle be …

The construction of microbial cell factories for sustainable production of chemicals and
pharmaceuticals requires extensive genome engineering. Using Saccharomyces cerevisiae …

Genome engineering based on homologous recombination has been applied to yeast for
many years. However, the growing importance of yeast as a cell factory in metabolic …

The DNA technologies developed over the past 20 years for reading and writing the genetic
code converged when the first synthetic cell was created 4 years ago. An outcome of this …