In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

Superoxide, O2•−, is formed in all living organisms that come in contact with air, and,
depending upon its biological context, it may act as a signaling agent, a toxic species, or a …

Nuclear magnetic resonance (NMR) spectroscopy is a well-established method for
analyzing protein structure, interaction, and dynamics at atomic resolution and in various …

A detailed knowledge of the complex processes that make cells and organisms alive is
fundamental in order to understand diseases and to develop novel drugs and therapeutic …

Protein misfolding and aggregation, a key contributor to the progression of numerous
neurodegenerative diseases, results in functional deficiencies and the creation of harmful …

NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

We use NMR directly in live human cells to describe the complete post-translational
maturation process of human superoxide dismutase 1 (SOD1). We follow, at atomic …

Recent advances in NMR methodology and techniques allow the structural investigation of
biomolecules of increasing size with atomic resolution. NMR spectroscopy is especially well …

Classical structural biology approaches allow structural characterization of biological
macromolecules in vitro, far from their physiological context. Nowadays, thanks to the wealth …

Abstract Superoxide dismutase-1 (SOD1) mutants, including those with unaltered enzymatic
activity, are known to cause amyotrophic lateral sclerosis (ALS). Several destabilizing factors …