Artificial cells are constructed to imitate natural cells and allow researchers to explore
biological process and the origin of life. The construction methods for artificial cells, through …

Synthetic genomics is the construction of viruses, bacteria, and eukaryotic cells with
synthetic genomes. It involves two basic processes: synthesis of complete genomes or …

For decades, identifying the regions of a bacterial chromosome that are necessary for
viability has relied on mapping integration sites in libraries of random transposon mutants to …

Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …

Dangerous, antibiotic resistant bacteria have been observed with increasing frequency over
the past several decades. In this review the factors that have been linked to this …

INTRODUCTION In 1984, the simplest cells capable of autonomous growth, the
mycoplasmas, were proposed as models for understanding the basic principles of life. In …

Since 2006, numerous cases of bacterial symbionts with extraordinarily small genomes
have been reported. These organisms represent independent lineages from diverse …

We report the design, synthesis, and assembly of the 1.08–mega–base pair Mycoplasma
mycoides JCVI-syn1. 0 genome starting from digitized genome sequence information and its …

Artificial cells have attracted much attention as substitutes for natural cells. There are many
different forms of artificial cells with many different definitions. They can be integral biological …

The pathways that comprise cellular metabolism are highly interconnected, and alterations
in individual enzymes can have far-reaching effects. As a result, global profiling methods …