CRISPR/Cas9 in genome editing and beyond
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …
regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a …
CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering
DJ Dickinson, B Goldstein - Genetics, 2016 - academic.oup.com
The advent of genome editing techniques based on the clustered regularly interspersed
short palindromic repeats (CRISPR)–Cas9 system has revolutionized research in the …
short palindromic repeats (CRISPR)–Cas9 system has revolutionized research in the …
The Caenorhabditis elegans Transgenic Toolbox
J Nance, C Frøkjær-Jensen - Genetics, 2019 - academic.oup.com
The power of any genetic model organism is derived, in part, from the ease with which gene
expression can be manipulated. The short generation time and invariant developmental …
expression can be manipulated. The short generation time and invariant developmental …
A CRISPR view of development
MM Harrison, BV Jenkins… - Genes & …, 2014 - genesdev.cshlp.org
The CRISPR (clustered regularly interspaced short palindromic repeat)–Cas9 (CRISPR-
associated nuclease 9) system is poised to transform developmental biology by providing a …
associated nuclease 9) system is poised to transform developmental biology by providing a …
Dramatic enhancement of genome editing by CRISPR/Cas9 through improved guide RNA design
B Farboud, BJ Meyer - Genetics, 2015 - academic.oup.com
Success with genome editing by the RNA-programmed nuclease Cas9 has been limited by
the inability to predict effective guide RNAs and DNA target sites. Not all guide RNAs have …
the inability to predict effective guide RNAs and DNA target sites. Not all guide RNAs have …
Conditional knockouts generated by engineered CRISPR-Cas9 endonuclease reveal the roles of coronin in C. elegans neural development
Conditional gene knockout animals are valuable tools for studying the mechanisms
underlying cell and developmental biology. We developed a conditional knockout strategy …
underlying cell and developmental biology. We developed a conditional knockout strategy …
Heat‐shock‐inducible CRISPR/Cas9 system generates heritable mutations in rice
Transient expression of CRISPR/Cas9 is an effective approach for limiting its activities and
improving its precision in genome editing. Here, we describe the heat‐shock‐inducible …
improving its precision in genome editing. Here, we describe the heat‐shock‐inducible …
CRISPR/Cas9 Mediates Efficient Conditional Mutagenesis in Drosophila
Z Xue, M Wu, K Wen, M Ren, L Long… - G3: Genes …, 2014 - academic.oup.com
Existing transgenic RNA interference (RNAi) methods greatly facilitate functional genome
studies via controlled silencing of targeted mRNA in Drosophila. Although the RNAi …
studies via controlled silencing of targeted mRNA in Drosophila. Although the RNAi …
MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo
X Zhang, S Lv, Z Luo, Y Hu, X Peng, J Lv… - Nucleic acids …, 2021 - academic.oup.com
CRISPR-mediated gene activation (CRISPRa) is a promising therapeutic gene editing
strategy without inducing DNA double-strand breaks (DSBs). However, in vivo …
strategy without inducing DNA double-strand breaks (DSBs). However, in vivo …
Mechanisms of precise genome editing using oligonucleotide donors
The use of programmable meganucleases is transforming genome editing and functional
genomics. CRISPR/Cas9 was developed such that targeted genomic lesions could be …
genomics. CRISPR/Cas9 was developed such that targeted genomic lesions could be …