Gram-negative bacteria possess an outer membrane layer which constrains uptake and
secretion of solutes and polypeptides. To overcome this barrier, bacteria have developed …

Cell-surface display allows peptides and proteins to be displayed on the surface of microbial
cells by fusing them with the anchoring motifs. The protein to be displayed–the passenger …

Autotransport in Gram-negative bacteria denotes the ability of surface-localized proteins to
cross the outer membrane (OM) autonomously. Autotransporters perform this task with the …

The autotransporters, a family of secreted proteins from Gram-negative bacteria, possess an
overall unifying structure comprising three functional domains: the amino-terminal leader …

Controlled human malaria infection (CHMI) entails deliberate infection with malaria
parasites either by mosquito bite or by direct injection of sporozoites or parasitized …

Autotransporters are virulence‐related proteins of Gram‐negative bacteria that are secreted
via an outer‐membrane‐based C‐terminal extension, the translocator domain. This domain …

Pathogenicity islands are chromosomal clusters of pathogen-specific virulence genes often
found at tRNA loci. We have determined the molecular genetic structure of SPI-3, a 17-kb …

Display of heterologous proteins on the surface of microorganisms, enabled by means of
recombinant DNA technology, has become an increasingly used strategy in various …

In recent years, the use of surface-display vectors for displaying polypeptides on the surface
of bacteriophage and bacteria, combined with in vitro selection technologies, has …

Despite the first report on the bacterial display of a recombinant peptide appeared almost 30
years ago, industrial application of cells with surface-displayed enzymes is still limited. To …