Supplementation of glial cell line-derived neurotrophic factor, fibroblast growth factor 2, and epidermal growth factor promotes self-renewal of putative buffalo (Bubalus …

A Sharma, SV Lagah, D Nagoorvali… - Cellular …, 2019 - liebertpub.com
In this study, we investigated the effect of glial cell line-derived neurotrophic factor (GDNF),
fibroblast growth factor (FGF) 2, and epidermal growth factor (EGF) on the expression of …

Propagation of goat putative spermatogonial stem cells under growth factors defined serum-free culture conditions

A Sharma, SM Shah, M Tiwari, M Roshan, MK Singh… - Cytotechnology, 2020 - Springer
In the present study, we used a serum-free culture media to propagate goat putative
spermatogonial stem cells (SSCs) and evaluated the effect of crucial growth factors on …

Optimization of Serum-Free Culture Conditions for Propagation of Putative Buffalo (Bubalus bubalis) Spermatogonial Stem Cells

A Sharma, SM Shah, N Saini, P Mehta… - Cellular …, 2019 - liebertpub.com
Spermatogonial stem cells (SSCs) self-renew and produce a large number of differentiated
germ cells to maintain normal spermatogenesis. However, the growth factors crucial for SSC …

Successful transplantation of transfected enriched buffalo (Bubalus bubalis) spermatogonial stem cells to homologous recipients

A Sharma, A Kumaresan, P Mehta, N Nala, MK Singh… - Theriogenology, 2020 - Elsevier
Genetic modification of spermatogonial stem cells (SSCs) is an alternative method to
pronuclear microinjection and somatic cell nuclear transfer for transgenesis in large animals …

Spermatogonial stem cells: their use in fertility restoration

A Sharma, A Kumaresan, P Vats… - Frontier Technologies in …, 2022 - Springer
Spermatogonial stem cells (SSCs) are the only adult stem cells in males capable of
transmitting their genetic information to the next generation. Over the past two decades …

[PDF][PDF] Transcriptome sequencing analysis of goat testicular tissue

Q He, Z Zhao, Z Dai, L Qiao, T Yang - Indian Journal Animal Health, 2019 - ijah.in
Testicular development and spermatogenesis play vital roles in goat reproduction and
production. However, only a minimal quantity of genomic information is available on goat …

[PDF][PDF] Homologous transplantation of fluorescently labelled enriched buffalo (Bubalus bubalis) spermatogonial stem cells to prepubertal recipients

A Sharma, A Kumaresan, N Nala, M Tiwari… - Indian J Anim …, 2020 - epubs.icar.org.in
Spermatogonial stem cell transplantation provides a unique opportunity to study the biology
of spermatogenesis and also offers an alternative approach for genetic modification in large …

Development of spermatogonial stem cell niche and immunoexpression of vimentin filaments in the testes of prenatal and postnatal Indian buffalo (Bubalus bubalis)

AQ Carlo, D Pathak, O Singh… - Turkish Journal of …, 2019 - journals.tubitak.gov.tr
In this study, we established the components of the buffalo spermatogonial stem cell (SSC)
niche in prenatal (38.5-280 days of gestation), neonatal, prepubertal, and adult testes …

Generation of ETV5 knockout pigs with CRISPR/Cas9

M Zhang, G Cai, R Zhou, H Yang - Indian Journal of Animal …, 2021 - indianjournals.com
Background Ets variant factor 5 (ETV5) plays an important regulatory role in mouse
spermatogonial stem cells (SSCs) self-renewal. ETV5 knockout (KO) mice exhibit a …

Effect of stage of puberty on culture characteristics of goat spermatogonial stem cells

M Pathak, SD Kharche, SP Singh… - Indian Journal of …, 2021 - indianjournals.com
The aim of experiment was to study the culture characteristics of spermatogonial stem cells
(SSCs) obtained from pre-and post-pubertal goat testes. Six animals each of two age groups …