Coupled transcription-translation (CTT) is a hallmark of prokaryotic gene expression. CTT
occurs when ribosomes associate with and initiate translation of mRNAs whose transcription …

Lipids and the membranes they form are fundamental building blocks of cellular life, and
their geometry and chemical properties distinguish membranes from other cellular …

Once described as mere “bags of enzymes,” bacterial cells are in fact highly organized, with
many macromolecules exhibiting nonuniform localization patterns. Yet the physical and …

Counting molecules in complexes is challenging, even with super-resolution microscopy.
Here, we use the programmable and specific binding of dye-labeled DNA probes to count …

Despite the fundamental importance of transcription, a comprehensive analysis of RNA
polymerase (RNAP) behavior and its role in the nucleoid organization in vivo is lacking …

Localization-based super-resolution techniques open the door to unprecedented analysis of
molecular organization. This task often involves complex image processing adapted to the …

Dense fluorophore labeling without compromising the biological target is crucial for genuine
super-resolution microscopy. Here we introduce a broadly applicable labeling strategy for …

Zu dem Thema gibt es viele Publikationen, die von Experten für Experten geschrieben
wurden. Dieses Buch wendet sich insbesondere an Studenten höherer Semester und …

The precise molecular architecture of synaptic active zones (AZs) gives rise to different
structural and functional AZ states that fundamentally shape chemical neurotransmission …

Single-molecule localization microscopy (SMLM) generates super-resolution images by
serially detecting individual fluorescent molecules. The power of SMLM, however, goes …