BACKGROUND In humans, normal healthy children are regularly produced through
fertilization of fresh oocytes with fresh spermatozoa. However, asynchrony between oocytes …

Somatic cell nuclear transfer (SCNT) has been an area of interest in the field of stem cell
research and regenerative medicine for the past 20 years. The main biological goal of SCNT …

During differentiation, somatic nuclei acquire highly specialized DNA and chromatin
modifications, which are thought to result in cellular memory of the differentiated state [1] …

The present study demonstrated that brief treatment of in vitro-matured porcine oocytes with
demecolcine results in a membrane protrusion that contains a condensed chromosome …

Somatic cell nuclear transfer (SCNT) offers great potential for developing better animal
models of human disease. The domestic ferret (Mustela putorius furo) is an ideal animal …

Cloning of buffalos (Bubalus bubalis) through nuclear transfer is a potential alternative
approach in genetic improvement of buffalos. However, to our knowledge, cloned offspring …

Several studies have shown that both quiescent and proliferating somatic donor cells can be
fully reprogrammed after nuclear transfer (NT) and result in viable offspring. So far, however …

It is now over a decade since the birth, in 1996, of Dolly the first animal to be produced by
nuclear transfer using an adult derived somatic cell as nuclear donor. Since this time similar …

Somatic cell nuclear transfer was used to produce live piglets from cultured fetal fibroblast
cells. This was achieved by exposing donor cell nuclei to oocyte cytoplasm for …

In 1997, Wilmut et al. announced the birth of Dolly, the first ever clone of an adult animal. To
date, adult sheep, goats, cattle, mice, pigs, cats and rabbits have been cloned using somatic …