The lignin‐degrading abilities of Gelatoporia subvermispora gat1 and pex1 mutants generated via CRISPR/Cas9

T Nakazawa, C Inoue, R Morimoto… - Environmental …, 2023 - Wiley Online Library
White‐rot fungi efficiently degrade wood lignin; however, the mechanisms involved remain
largely unknown. Recently, a forward genetics approach to identify several genes in …

Experimental evidence that lignin‐modifying enzymes are essential for degrading plant cell wall lignin by Pleurotus ostreatus using CRISPR/Cas9

T Nakazawa, I Yamaguchi, Y Zhang… - Environmental …, 2023 - Wiley Online Library
Abstract Lignin‐modifying enzymes (LMEs), which include laccases (Lacs), manganese
peroxidases (MnPs), versatile peroxidases (VPs), and lignin peroxidases (LiPs), have been …

Pleurotus ostreatus as a model mushroom in genetics, cell biology, and material sciences

T Nakazawa, M Kawauchi, Y Otsuka, J Han… - Applied Microbiology …, 2024 - Springer
Pleurotus ostreatus, also known as the oyster mushroom, is a popular edible mushroom
cultivated worldwide. This review aims to survey recent progress in the molecular genetics of …

Genome sequence of the oyster mushroom Pleurotus ostreatus strain PC9

YY Lee, G Vidal-Diez de Ulzurrun, EM Schwarz… - G3, 2021 - academic.oup.com
The oyster mushroom Pleurotus ostreatus is a basidiomycete commonly found in the rotten
wood and it is one of the most cultivated edible mushrooms globally. Pleurotus ostreatus is …

Gene targeting of dikaryotic Pleurotus ostreatus nuclei using the CRISPR/Cas9 system

F Yamasaki, T Nakazawa, M Oh, D Bao… - FEMS Microbiology …, 2022 - academic.oup.com
Clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated
protein 9 (Cas9)-assisted gene targeting is a promising method used in molecular breeding …

Dominant effects of gat1 mutations on the ligninolytic activity of the white-rot fungus Pleurotus ostreatus

T Nakazawa, R Morimoto, H Wu, R Kodera… - Fungal biology, 2019 - Elsevier
In nature, white-rot fungi efficiently degrade lignin present in wood biomass. Elucidation of
molecular mechanisms underlying wood lignin biodegradation by white-rot fungi would …

Visualizing organelles with recombinant fluorescent proteins in the white-rot fungus Pleurotus ostreatus

K Kurebayashi, T Nakazawa, Y Higashitarumizu… - Fungal Biology, 2023 - Elsevier
White-rot fungi secrete numerous enzymes involved in lignocellulose degradation. However,
the secretory mechanisms or pathways, including protein synthesis, folding, modification …

Introducing multiple-gene mutations in Pleurotus ostreatus using a polycistronic tRNA and CRISPR guide RNA strategy

H Xu, T Nakazawa, Y Zhang, M Oh… - FEMS Microbiology …, 2022 - academic.oup.com
The white-rot fungus Pleurotus ostreatus is an agaricomycete that is frequently used in
molecular genetics studies as many useful tools are applicable to the fungus. In particular …

Comparative transcriptional analyses of Pleurotus ostreatus mutants on beech wood and rice straw shed light on substrate-biased gene regulation

H Wu, T Nakazawa, H Xu, R Yang, D Bao… - Applied Microbiology …, 2021 - Springer
Distinct wood degraders occupying their preferred habitats have biased enzyme repertoires
that are well fitted to their colonized substrates. Pleurotus ostreatus, commonly found on …

[PDF][PDF] Omic tools to study enzyme production from fungi in the Pleurotus genus.

M Téllez-Téllez, G Díaz-Godínez - BioResources, 2019 - bioresources.cnr.ncsu.edu
Fungi from the Pleurotus genus secrete different enzymes, including laccases, manganese
peroxidases, versatile peroxidases, glycosyl hydrolases, peptidases, and esterases/lipases …