Tuberculosis (TB) is among the most difficult infections to treat, requiring several months of
multidrug therapy to produce a durable cure. The reasons necessitating long treatment times …

Antibacterial agents target the products of essential genes but rarely achieve complete
target inhibition. Thus, the all-or-none definition of essentiality afforded by traditional genetic …

Essential genes encode the processes that are necessary for life. Until recently, commonly
applied binary classifications left no space between essential and non-essential genes. In …

The emergence of multidrug-resistant Gram-negative bacteria underscores the need to
define genetic vulnerabilities that can be therapeutically exploited. The Gram-negative …

ABSTRACT CRISPR interference (CRISPRi) has facilitated the study of essential genes in
diverse organisms using both high-throughput and targeted approaches. Despite the …

Microbiology and synthetic biology depend on reverse genetic approaches to manipulate
bacterial genomes; however, existing methods require molecular biology to generate …

Engineering metabolism to efficiently produce chemicals from multi-step pathways requires
optimizing multi-gene expression programs to achieve enzyme balance. CRISPR-Cas …

Functional genomics is the use of systematic gene perturbation approaches to determine the
contributions of genes under conditions of interest. Although functional genomic strategies …

Mycobacterium tuberculosis remains the most common infectious killer worldwide despite
decades of antitubercular drug development. Effectively controlling the tuberculosis (TB) …

During steady‐state cell growth, individual enzymatic fluxes can be directly inferred from
growth rate by mass conservation, but the inverse problem remains unsolved. Perturbing the …