P450BM3 (CYP102A1), a fatty acid hydroxylase from Bacillus megaterium, has been
extensively studied over a period of almost forty years. The enzyme has been redesigned to …

The performance of currently available minibioreactors with volumes below about 100 ml is
reviewed. Bioreactors are characterized by their area of application, by mass transfer and …

Cytochrome P450 ΒΜ-3 from Bacillus megaterium was engineered using a combination of
directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio-and …

Oxidations are key reactions in chemical syntheses. Biooxidations using fermentation
processes have already conquered some niches in industrial oxidation processes since they …

Drug metabolism in human liver is a process involving many different enzymes. Among
them, a number of cytochromes P450 isoforms catalyze the oxidation of most of the drugs …

Plant cytochrome P450 monooxygenases were long considered to be highly substrate-
specific, regioselective and stereoselective enzymes, in this respect differing from their …

Cytochrome P450 BM3 monooxygenase is the topic of extensive research as many
researchers have evolved this enzyme to generate a variety of products. However, the …

This paper reports on the application of the molecular Lego approach to P450 enzymes.
Protein domains are used as catalytic (P450 BM3 haem domain and human P450 2E1) or …

Over the past 20 years, biosensors have revolutionised the care and management of
diabetes and have had important impacts in several other areas of clinical diagnostics. This …

Cytochrome P450 enzymes are renowned for their ability to insert oxygen into an enormous
variety of compounds with a high degree of chemo-and regio-selectivity under mild …