Site-selective protein modification is of significant interest in chemical biology research, with
lysine residues representing a particularly challenging target. Whilst lysines are popular for …

Sortase‐mediated ligation (SML) has emerged as a powerful and versatile methodology for
site‐specific protein conjugation, functionalization/labeling, immobilization, and design of …

There are many efficient ways to connect proteins at termini. However, connecting at a loop
is difficult because of lower flexibility and variable environment. Here, we have developed …

Highlights•Sortase covalently links surface proteins and pili with the CWSS to
peptidoglycan.•Unfolded precursors of surface proteins and pili are folded non oxidatively or …

Gram-positive bacteria assemble pili (fimbriae) on their surfaces to adhere to host tissues
and to promote polymicrobial interactions. These hair-like structures, although very thin (1 to …

Many species of pathogenic gram‐positive bacteria display covalently crosslinked protein
polymers (called pili or fimbriae) that mediate microbial adhesion to host tissues. These …

Gram-positive bacteria display pili whose protein components (pilins) are covalently
crosslinked by pilus-specific sortase enzymes. These cysteine transpeptidase enzymes …

Pathogenic Gram-positive bacteria cause a range of serious infections in humans and
represent a significant threat to global health. The rising emergence of virulent strains which …

We developed both membrane potential nanosensors (MPNs) and voltage sensing dyes
(VSDs), and quantitative optical measurement tools to measure membrane potential (MP) at …