Light-sheet fluorescent microscopy: fundamentals, developments and applications

H Kafian, S Mozaffari-Jovin, M Bagheri… - Physica …, 2023 - iopscience.iop.org
Deep volumetric microscopy of live objects plays a critical role in biology and medicine. To
this end, development of rapid and non-invasive optical methods for 3-dimensional (3D) …

minispim—A miniaturized light-sheet microscope

PN Hedde - ACS sensors, 2021 - ACS Publications
The miniSPIM is a miniaturized light-sheet microscope that enables imaging with optical
sectioning on mobile camera devices such as smartphones and single-board computers …

A polymer index-matched to water enables diverse applications in fluorescence microscopy

X Han, Y Su, H White, KM O'Neill, NY Morgan… - Lab on a Chip, 2021 - pubs.rsc.org
We demonstrate diffraction-limited and super-resolution imaging through thick layers (tens-
hundreds of microns) of BIO-133, a biocompatible, UV-curable, commercially available …

Visualization of barriers and obstacles to molecular diffusion in live cells by spatial pair-cross-correlation in two dimensions

L Malacrida, PN Hedde, S Ranjit… - Biomedical optics …, 2017 - opg.optica.org
Despite recent advances in optical super-resolution, we lack a method that can visualize the
path followed by diffusing molecules in the cytoplasm or in the nucleus of cells …

Membrane remodeling by arc/Arg3. 1

PN Hedde, L Malacrida, B Barylko, DD Binns… - Frontiers in Molecular …, 2021 - frontiersin.org
The activity-regulated cytoskeletal-associated protein (Arc, also known as Arg3. 1) is an
immediate early gene product induced by activity/experience and required for multiple …

Single-molecule light-sheet microscopy with local nanopipette delivery

B Li, A Ponjavic, WH Chen, L Hopkins… - Analytical …, 2021 - ACS Publications
The detection of single molecules in biological systems has rapidly increased in resolution
over the past decade. However, the delivery of single molecules remains to be a challenge …

Photocell-Based Optofluidic Device for Clogging-Free Cell Transit Time Measurements

F Storti, S Bonfadini, G Bondelli, V Vurro, G Lanzani… - Biosensors, 2024 - mdpi.com
Measuring the transit time of a cell forced through a bottleneck is one of the most widely
used techniques for the study of cell deformability in flow. It in turn provides an accessible …

Integrative quantitative-phase and airy light-sheet imaging

NR Subedi, PS Jung, EL Bredeweg, S Nemati… - Scientific reports, 2020 - nature.com
Light-sheet microscopy enables considerable speed and phototoxicity gains, while
quantitative-phase imaging confers label-free recognition of cells and organelles, and …

SPIM-Flow: An Integrated Light Sheet and Microfluidics Platform for Hydrodynamic Studies of Hydra

PN Hedde, BT Le, EL Gomez, L Duong, RE Steele… - Biology, 2023 - mdpi.com
Simple Summary This work demonstrates an inexpensive light sheet microscope that can be
readily integrated with modern microfluidics. The integration between these two …

Sensitive light-sheet microscopy in multiwell plates using an AFM cantilever

A Ponjavic, Y Ye, E Laue, SF Lee… - Biomedical Optics …, 2018 - opg.optica.org
We present a sensitive inverted light sheet microscope, capable of single-molecule
fluorescence imaging of cells in 96-well plates. Light sheet microscope designs are often …