Spermatozoa cryopreservation: state of art and future in small ruminants

C Lv, G Wu, Q Hong, G Quan - Biopreservation and biobanking, 2019 - liebertpub.com
The cryotolerance of farm animal spermatozoa varies according to their specific features,
such as size, shape, and lipid composition. Thus, it is impossible to develop a standardized …

Extend the survival of human sperm in vitro in non-freezing conditions: damage mechanisms, preservation technologies, and clinical applications

Q Cheng, L Li, M Jiang, B Liu, Y Xian, S Liu, X Liu… - Cells, 2022 - mdpi.com
Preservation of human spermatozoa in vitro at normothermia or hypothermia maintaining
their functions and fertility for several days plays a significant role in reproductive biology …

Vitrification and conventional freezing methods in sperm cryopreservation: A systematic review and meta-analysis

Y Li, L Zhou, M Lv, P Ge, Y Liu, D Zhou - European Journal of Obstetrics & …, 2019 - Elsevier
Objective The objective is to systematically review and synthesize the literature on the
efficacy with two different cryopreservation methods used for human spermatozoa and …

A comprehensive review and update on human fertility cryopreservation methods and tools

S Antonouli, V Di Nisio, C Messini… - Frontiers in Veterinary …, 2023 - frontiersin.org
The broad conceptualization of fertility preservation and restoration has become already a
major concern in the modern western world since a large number of individuals often face it …

The impact of sperm DNA fragmentation on ICSI outcome in cases of donated oocytes

S Antonouli, A Papatheodorou, Y Panagiotidis… - Archives of gynecology …, 2019 - Springer
Purpose The aim of this study is to evaluate the sperm DNA fragmentation index (DFI) in
oocyte donation cycles and correlate it with the sperm parameters, the male characteristics …

[PDF][PDF] Simulated genital tract fluids and their applicability in drug release/dissolution testing of vaginal dosage forms

K Tietz, S Klein - Dissolut. Technol, 2018 - researchgate.net
There has been a trend towards the development of novel vaginal dosage forms both for
local therapy and systemic absorption. The growing number of vaginal dosage forms …

Technologies for Cryoprotectant-Free Vitrification of Human Spermatozoa: Asepticity as a Criterion for Effectiveness

V Isachenko, G Rahimi, P Mallmann, R Sanchez… - In Vitro Fertilization: A …, 2019 - Springer
This review describes the 120-year history of technology for cryoprotectant-free
cryopreservation of human spermatozoa by direct plunging into liquid nitrogen (vitrification) …

When condensed matter physics meets biology: Does superhydrophobicity benefiting the cryopreservation of human spermatozoa?

KD Esmeryan, Y Lazarov, GS Stamenov, TA Chaushev - Cryobiology, 2020 - Elsevier
With the increasing demand in regenerative and reproductive medicine for successful
conservation of living matter, the need of reliable platform in cell banking seems inevitable …

Cryopreservation of low number of human spermatozoa; which is better: vapor phase or direct submerging in liquid nitrogen?

A Hosseini, MA Khalili, AR Talebi, A Agha-Rahimi… - Human …, 2019 - Taylor & Francis
Cryopreservation and subsequent survival of semen samples with low numbers of human
spermatozoa in assisted reproductive technology (ART) facilities can be challenging. The …

Single sperm vitrification with permeable cryoprotectant-free medium is more effective in patients with severe oligozoospermia and azoospermia

B Maleki, MA Khalili, L Gholizadeh, E Mangoli… - Cryobiology, 2022 - Elsevier
Testicular sperm extraction (TESE) is an invasive surgery for achieving the spermatozoa in
cases with azoospermia. In these patients, the number of retrieved spermatozoa is limited …