A transcriptome constructed from short-read RNA sequencing (RNA-seq) is an easily
attainable proxy catalog of protein-coding genes when genome assembly is unnecessary …

Advances in transcriptome sequencing allow for simultaneous interrogation of differentially
expressed genes from multiple species originating from a single RNA sample, termed dual …

Abstract SPAdes—St. Petersburg genome Assembler—was originally developed for de
novo assembly of genome sequencing data produced for cultivated microbial isolates and …

The increasing application of RNA sequencing to study non-model species demands easy-
to-use and efficient bioinformatics tools to help researchers quickly uncover biological and …

Many of the major biological discoveries of the 20th century were made using just six
species: Escherichia coli bacteria, Saccharomyces cerevisiae and Schizosaccharomyces …

Background Biomedical researchers use alignments produced by BLAST (Basic Local
Alignment Search Tool) to categorize their query sequences. Producing such alignments is …

Metagenomics and total RNA sequencing (total RNA-Seq) have the potential to improve the
taxonomic identification of diverse microbial communities, which could allow for the …

In early January 2020, the novel coronavirus (SARS-CoV-2) responsible for a pneumonia
outbreak in Wuhan, China, was identified using next-generation sequencing (NGS) and …

Complex statistical methods are continuously developed across the fields of ecology,
evolution, and systematics (EES). These fields, however, lack standardized principles for …

Significant innovations in next-generation sequencing techniques and bioinformatics tools
have impacted our appreciation and understanding of RNA. Practical RNA sequencing …