Nucleic acid amplification has permeated every field in the life sciences since the
introduction of the classic polymerase chain reaction (PCR) method in 1983. Yet, despite its …

Recombinase polymerase amplification (RPA) is a highly sensitive and selective isothermal
amplification technique, operating at 37–42° C, with minimal sample preparation and …

BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has
stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them …

Although a fundamental understanding of the pathogenicity of most biothreat agents has
been elucidated and available treatments have increased substantially over the past …

Isothermal molecular diagnostics are bridging the technology gap between traditional
diagnostics and polymerase chain reaction-based methods. These new techniques enable …

As a conventional amplification technique, polymerase chain reaction (PCR) has been
widely applied to detect a variety of analytes with exponential amplification efficiency …

Multiplexed isothermal amplification and detection of nucleic acid sequences and
biomarkers is of increasing importance in diverse areas including advanced diagnostics …

Background Microfluidic chip detection technology is considered a potent tool for many
bioanalytic applications. Rapid detection of foodborne pathogens in the early stages is …

ABSTRACT A method for the rapid diagnosis of early dengue virus (DENV) infection is
highly needed. Here, a prototype reverse transcription-recombinase polymerase …

Nucleic acid amplification tests (NAATs) are very sensitive and specific methods, but they
mainly rely on centralized laboratories and therefore are not suitable for point-of-care …