The cystine knot structural motif is present in peptides and proteins from a variety of species,
including fungi, plants, marine molluscs, insects and spiders. It comprises an embedded ring …

Among the pathways used by gram-negative bacteria for protein secretion, the
autotransporter pathway represents a solution of impressive simplicity. Proteins are …

Recent improvements in bacterial surface display systems coupled with efficient selection
and screening strategies are propelling bacterial display systems to the forefront of peptide …

There is a critical need for compounds that target cell surface integrin receptors for
applications in cancer therapy and diagnosis. We used directed evolution to engineer the …

In this article, we will first introduce the squash inhibitors, a well established family of highly
potent canonical serine proteinase inhibitors isolated from Cucurbitaceae. The squash …

Flow cytometry is a powerful, high-throughput library screening tool in numerous
applications including the isolation of bioactive molecules from synthetic combinatorial …

A variety of expression systems for the display of either short peptides or fully folded proteins
on E. coli and, to a lesser extent, on Gram‐positive bacteria have been developed. The …

To display a protein or peptide with a distinct function at the surface of a living bacterial cell
is a challenging exercise with constantly increasing impact in many areas of biochemistry …

Hyperpolarization of small peptides by parahydrogen-induced polarization (PHIP) to
increase the sensitivity of nuclear magnetic resonance (NMR) techniques is well …

Cystine‐knot miniproteins are members of a large family of small proteins that are defined by
a common structural scaffold which is stabilized by three intramolecular disulfide bonds …