Rapid and accurate profiling of infection-causing pathogens remains a significant challenge
in modern health care. Despite advances in molecular diagnostic techniques, blood culture …

The primary clinical manifestations of paroxysmal nocturnal hemoglobinuria (PNH) are
hemolytic anemia, marrow failure, and thrombophilia. However, PNH is not a simple binary …

Precise genome-editing relies on the repair of sequence-specific nuclease-induced DNA
nicking or double-strand breaks (DSBs) by homology-directed repair (HDR). However …

Background: High-resolution amplicon melting analysis was recently introduced as a closed-
tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003; 49: 396 …

Infectious diseases are a major global cause of morbidity and mortality, seriously affecting
public health and socioeconomic stability. Since infectious diseases can be caused by a …

High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and
sequence matching. The melting profile of a PCR product depends on its GC content, length …

Background: High-resolution melting of PCR amplicons with the DNA dye LCGreen™ I was
recently introduced as a homogeneous, closed-tube method of genotyping that does not …

Plant mutagenesis is rapidly coming of age in the aftermath of recent developments in high‐
resolution molecular and biochemical techniques. By combining the high variation of …

Background: Screening for heterozygous sequence changes in PCR products, also known
as “mutation scanning”, is an important tool for genetic research and clinical applications …

Bacterial strain typing, or identifying bacteria at the strain level, is particularly important for
diagnosis, treatment, and epidemiological surveillance of bacterial infections. This is …