Assessing and advancing the safety of CRISPR-Cas tools: from DNA to RNA editing
CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …
decade and holds great promise for the treatment of human genetic diseases. Here we …
DNA double-strand break repair-pathway choice in somatic mammalian cells
The major pathways of DNA double-strand break (DSB) repair are crucial for maintaining
genomic stability. However, if deployed in an inappropriate cellular context, these same …
genomic stability. However, if deployed in an inappropriate cellular context, these same …
[HTML][HTML] Patterns of somatic structural variation in human cancer genomes
A key mutational process in cancer is structural variation, in which rearrangements delete,
amplify or reorder genomic segments that range in size from kilobases to whole …
amplify or reorder genomic segments that range in size from kilobases to whole …
[HTML][HTML] Mapping the genetic landscape of DNA double-strand break repair
Cells repair DNA double-strand breaks (DSBs) through a complex set of pathways critical for
maintaining genomic integrity. To systematically map these pathways, we developed a high …
maintaining genomic integrity. To systematically map these pathways, we developed a high …
Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites
CRISPR/Cas9-based genome editing has revolutionized experimental molecular biology
and entered the clinical world for targeted gene therapy. Identifying DNA modifications …
and entered the clinical world for targeted gene therapy. Identifying DNA modifications …
Transcription-mediated replication hindrance: a major driver of genome instability
B Gómez-González, A Aguilera - Genes & development, 2019 - genesdev.cshlp.org
Genome replication involves dealing with obstacles that can result from DNA damage but
also from chromatin alterations, topological stress, tightly bound proteins or non-B DNA …
also from chromatin alterations, topological stress, tightly bound proteins or non-B DNA …
Multiple roles of DNA2 nuclease/helicase in DNA metabolism, genome stability and human diseases
L Zheng, Y Meng, JL Campbell… - Nucleic acids research, 2020 - academic.oup.com
DNA2 nuclease/helicase is a structure-specific nuclease, 5′-to-3′ helicase, and DNA-
dependent ATPase. It is involved in multiple DNA metabolic pathways, including Okazaki …
dependent ATPase. It is involved in multiple DNA metabolic pathways, including Okazaki …
Gene duplication and deletion caused by over-replication at a fork barrier
J Oehler, CA Morrow, MC Whitby - Nature communications, 2023 - nature.com
Replication fork stalling can provoke fork reversal to form a four-way DNA junction. This
remodelling of the replication fork can facilitate repair, aid bypass of DNA lesions, and …
remodelling of the replication fork can facilitate repair, aid bypass of DNA lesions, and …
Yeast EndoG prevents genome instability by degrading extranuclear DNA species
In metazoans mitochondrial DNA (mtDNA) or retrotransposon cDNA released to cytoplasm
are degraded by nucleases to prevent sterile inflammation. It remains unknown whether …
are degraded by nucleases to prevent sterile inflammation. It remains unknown whether …
Mechanisms of insertions at a DNA double-strand break
Insertions and deletions (indels) are common sources of structural variation, and insertions
originating from spontaneous DNA lesions are frequent in cancer. We developed a highly …
originating from spontaneous DNA lesions are frequent in cancer. We developed a highly …