Nucleic acid extraction techniques and application to the microchip

CW Price, DC Leslie, JP Landers - Lab on a Chip, 2009 - pubs.rsc.org
As recently as the early 1990s, DNA purification was time-consuming, requiring the use of
toxic, hazardous reagents. The advent of solid phase extraction techniques and the …

based sample-to-answer molecular diagnostic platform for point-of-care diagnostics

JR Choi, R Tang, SQ Wang, WABW Abas… - Biosensors and …, 2015 - Elsevier
Nucleic acid testing (NAT), as a molecular diagnostic technique, including nucleic acid
extraction, amplification and detection, plays a fundamental role in medical diagnosis for …

Current trends of microfluidic single-cell technologies

P Shinde, L Mohan, A Kumar, K Dey, A Maddi… - International journal of …, 2018 - mdpi.com
The investigation of human disease mechanisms is difficult due to the heterogeneity in gene
expression and the physiological state of cells in a given population. In comparison to bulk …

Applications of microfluidics for molecular diagnostics

H Jayamohan, HJ Sant, BK Gale - Microfluidic Diagnostics: Methods and …, 2013 - Springer
Diagnostic assays implemented in microfluidic devices have developed rapidly over the past
decade and are expected to become commonplace in the next few years. Hundreds of …

Characterization of dynamic solid phase DNA extraction from blood with magnetically controlled silica beads

GRM Duarte, CW Price, JL Littlewood, DM Haverstick… - Analyst, 2010 - pubs.rsc.org
A novel solid phase extraction technique is described where DNA is bound and eluted from
magnetic silica beads in a manner where efficiency is dependent on the magnetic …

A facile one-step method for cell lysis and DNA extraction of waterborne pathogens using a microchip

V Kamat, S Pandey, K Paknikar, D Bodas - Biosensors and Bioelectronics, 2018 - Elsevier
Globally, waterborne organisms are the primary causative agents for the transmission of
various forms of diarrheal diseases. For accurate diagnosis, molecular tools have gained …

Integrated microfluidic systems for DNA analysis

SK Njoroge, HW Chen, MA Witek, SA Soper - Microfluidics: Technologies …, 2011 - Springer
The potential utility of genome-related research in terms of evolving basic discoveries in
biology has generated widespread use of DNA diagnostics and DNA forensics and driven …

Low cost fluorescence detection using a CCD array and image processing for on-chip gel electrophoresis

R Walczak, W Kubicki, J Dziuban - Sensors and Actuators B: Chemical, 2017 - Elsevier
The most commonly applied method of fluorescence detection for on-chip gel
electrophoresis is finish line detection in epifluorescence configuration using highly …

Microfluidic enzymatic DNA extraction on a hybrid polyester-toner-PMMA device

BL Thompson, C Birch, J Li, JA DuVall, D Le Roux… - Analyst, 2016 - pubs.rsc.org
To date, the forensic community regards solid phase extraction (SPE) as the most effective
methodology for the purification of DNA for use in short tandem repeat (STR) polymerase …

A radiation heating-based oscillatory polymerase chain reaction system for detecting donkey-hide gelatin

JJ Chen, TY Kuo, SC Sheu… - Journal of …, 2024 - asmedigitalcollection.asme.org
Polymerase chain reaction (PCR) is a biochemical technique for copying DNA by repeatedly
changing the temperature of nucleic acid samples. In this study, we aim to create an …