Fertilization calcium waves are introduced, and the evidence from which we can infer
general mechanisms of these waves is presented. The two main classes of hypotheses put …

The discovery of highly efficient gene targeting in Dictyostelium (De Lozanne and Spudich,
1987) and the use of the antisense RNA approach in this organism (Knecht and Loomis …

F-actin is the major component of muscle thin filaments and, more generally, of the
microfilaments of the dynamic, multifunctional cytoskeletal systems of nonmuscle eukaryotic …

We provide the first link between a known molecular motor and morphogenesis, the
fundamental process of cell shape changes and movements that characterizes development …

The role of myosin II in mitosis is generally thought to be restricted to cytokinesis. We present
surprising new evidence that cortical myosin II is also required for spindle assembly in cells …

The role of myosin in the contraction of striated muscle cells is well known, but its importance
in nonmuscle cells is not yet clear. The function of myosin in Dictyostelium discoideum has …

Two independent approaches to understanding the molecular mechanism of cytokinesls
have converged on the gene spaghetti-squash (sqh). A genetic screen for mltotlc mutants …

One of our monoclonal antibodies against the heavy chain of bovine kinesin (H2) also
recognized the heavy chain of squid kinesin. The immunofluorescence pattern of H2 in …

Mitotic spindles are microtubule-based structures responsible for chromosome partitioning
during cell division. Although the roles of microtubules and microtubule-based motors in …

The eukaryotic slime mold Dictyostelium discoideum has a single conventional myosin
heavy chain gene (mhcA). The elimination of the mhcA gene was achieved by homologous …