Precise spatio–temporal control of gene activity is essential for organismal development,
growth, and survival in a changing environment. Decisive steps in gene regulation involve …

The journey of RNA polymerase II (Pol II) as it transcribes a gene is anything but a smooth
ride. Transcript elongation is discontinuous and can be perturbed by intrinsic regulatory …

Metazoan gene regulation often involves the pausing of RNA polymerase II (Pol II) in the
promoter-proximal region. Paused Pol II is stabilized by the protein complexes DRB …

Abstract RNA polymerase II (Pol II) transcribes all protein-coding genes and many
noncoding RNAs in eukaryotic genomes. Although Pol II is a complex, 12-subunit enzyme, it …

Transcription is a highly dynamic process. Consequently, we have developed native
elongating transcript sequencing technology for mammalian chromatin (mNET-seq), which …

The programmes that direct an organism's development and maintenance are encoded in its
genome. Decoding of this information begins with regulated transcription of genomic DNA …

Regulation of the elongation phase of transcription by RNA polymerase II (Pol II) is utilized
extensively to generate the pattern of mRNAs needed to specify cell types and to respond to …

Nucleosomes are barriers to transcription in vitro; however, their effects on RNA polymerase
in vivo are unknown. Here we describe a simple and general strategy to comprehensively …

Chromatin modifications are associated with many aspects of gene expression, yet their role
in cellular transitions during development remains elusive. Here, we use a new approach to …

Elongation is becoming increasingly recognized as a critical step in eukaryotic
transcriptional regulation. Although traditional genetic and biochemical studies have …