The HEK293 cell line has earned its place as a producer of biotherapeutics. In addition to its
ease of growth in serum-free suspension culture and its amenability to transfection, this cell …

Introduction: Lentiviruses are a very potent class of viral vectors for which there is presently a
rapidly growing interest for a number of gene therapy. However, their construction …

We recently developed adeno-associated virus (AAV) capsids to facilitate efficient and
noninvasive gene transfer to the central and peripheral nervous systems. However, a …

Previous studies using B16BL6-derived exosomes labelled with gLuc–lactadherin (gLuc-
LA), a fusion protein of Gaussia luciferase (a reporter protein) and lactadherin (an exosome …

A hallmark of inflammasome activation is the ASC speck, a micrometre-sized structure
formed by the inflammasome adaptor protein ASC (apoptosis-associated speck-like protein …

Missense mutations in PTEN-induced kinase 1 (PINK1) cause autosomal-recessive
inherited Parkinson's disease (PD). We have exploited our recent discovery that …

Parkinson's disease predisposing LRRK2 kinase phosphorylates a group of Rab GTPase
proteins including Rab29, within the effector‐binding switch II motif. Previous work indicated …

Heart rate is under the precise control of the autonomic nervous system. However, the wiring
of peripheral neural circuits that regulate heart rate is poorly understood. Here, we develop a …

Type 1 diabetes is an autoimmune disease initiated by the invasion of pancreatic islets by
immune cells that selectively kill the β cells. We found that rodent and human T lymphocytes …

Adeno-associated viral (AAV) manufacturing at scale continues to hinder the application of
AAV technology to gene therapy studies. Although scalable systems based on AAV …