Biocatalysis has undergone revolutionary progress in the past century. Benefited by the
integration of multidisciplinary technologies, natural enzymatic reactions are constantly …

Digital Polymerase Chain Reaction (dPCR) is a novel method for the absolute quantification
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …

Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …

Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …

Polydimethylsiloxane (PDMS) is a dominant material in the fabrication of microfluidic
devices to generate water-in-oil droplets, particularly lipid-stabilized droplets, because of its …

There is a recognized and growing need for rapid and efficient cell assays, where the size of
microfluidic devices lend themselves to the manipulation of cellular populations down to the …

Designing catalysts that achieve the rates and selectivities of natural enzymes is a long-
standing goal in protein chemistry. Here, we show that an ultrahigh-throughput droplet …

The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

Ultrahigh-throughput screening, in which members of enzyme libraries compartmentalized
in water-in-oil emulsion droplets are assayed, has emerged as a powerful format for directed …

Droplet microfluidics is a valuable method to “beat the odds” in high throughput screening
campaigns such as directed evolution, where valuable hits are infrequent and large library …