Construction of a linker library with widely controllable flexibility for fusion protein design
Flexibility or rigidity of the linker between two fused proteins is an important parameter that
affects the function of fusion proteins. In this study, we constructed a linker library with five …
affects the function of fusion proteins. In this study, we constructed a linker library with five …
Natural compounds as therapeutic agents: The case of human topoisomerase ib
A Ottaviani, F Iacovelli, P Fiorani… - International Journal of …, 2021 - mdpi.com
Natural products are widely used as source for drugs development. An interesting example
is represented by natural drugs developed against human topoisomerase IB, a ubiquitous …
is represented by natural drugs developed against human topoisomerase IB, a ubiquitous …
[HTML][HTML] Topoisomerase IB: a relaxing enzyme for stressed DNA
BC Soren, JB Dasari, A Ottaviani, F Iacovelli… - Cancer Drug …, 2020 - ncbi.nlm.nih.gov
DNA topoisomerase I enzymes relieve the torsional strain in DNA; they are essential for
fundamental molecular processes such as DNA replication, transcription, recombination …
fundamental molecular processes such as DNA replication, transcription, recombination …
DNA topoisomerase I domain interactions impact enzyme activity and sensitivity to camptothecin
CM Wright, M Van Der Merwe, AH DeBrot… - Journal of Biological …, 2015 - ASBMB
During processes such as DNA replication and transcription, DNA topoisomerase I (Top1)
catalyzes the relaxation of DNA supercoils. The nuclear enzyme is also the cellular target of …
catalyzes the relaxation of DNA supercoils. The nuclear enzyme is also the cellular target of …
Simulations of DNA topoisomerase 1B bound to supercoiled DNA reveal changes in the flexibility pattern of the enzyme and a secondary protein–DNA binding site
Human topoisomerase 1B has been simulated covalently bound to a negatively supercoiled
DNA minicircle, and its behavior compared to the enzyme bound to a simple linear DNA …
DNA minicircle, and its behavior compared to the enzyme bound to a simple linear DNA …
Molecular mechanism of the camptothecin resistance of Glu710Gly topoisomerase IB mutant analyzed in vitro and in silico
C Tesauro, B Morozzo della Rocca, A Ottaviani… - Molecular cancer, 2013 - Springer
Background DNA topoisomerases are key enzymes that modulate the topological state of
DNA through the breaking and rejoining of DNA strands. Human topoisomerase IB can be …
DNA through the breaking and rejoining of DNA strands. Human topoisomerase IB can be …
In vitro and in silico characterization of an antimalarial compound with antitumor activity targeting human DNA topoisomerase IB
BC Soren, J Babu Dasari, A Ottaviani… - International Journal of …, 2021 - mdpi.com
Human DNA topoisomerase IB controls the topological state of supercoiled DNA through a
complex catalytic cycle that consists of cleavage and religation reactions, allowing the …
complex catalytic cycle that consists of cleavage and religation reactions, allowing the …
Binding of an Indenoisoquinoline to the topoisomerase-DNA complex induces reduction of linker mobility and strengthening of protein-DNA interaction
Long-duration comparative molecular dynamics simulations of the DNA-topoisomerase
binary and DNA-topoisomerase-indenoisoquinoline ternary complexes have been carried …
binary and DNA-topoisomerase-indenoisoquinoline ternary complexes have been carried …
Replacement of the human topoisomerase linker domain with the plasmodial counterpart renders the enzyme camptothecin resistant
B Arnò, I D'Annessa, C Tesauro, L Zuccaro… - PLoS …, 2013 - journals.plos.org
A human/plasmodial hybrid enzyme, generated by swapping the human topoisomerase IB
linker domain with the corresponding domain of the Plasmodium falciparum enzyme, has …
linker domain with the corresponding domain of the Plasmodium falciparum enzyme, has …
The human topoisomerase 1B Arg634Ala mutation results in camptothecin resistance and loss of inter-domain motion correlation
I D'Annessa, C Tesauro, Z Wang, B Arnò… - … et Biophysica Acta (BBA …, 2013 - Elsevier
Human topoisomerase 1B, the unique target of the natural anticancer compound
camptothecin, catalyzes the unwinding of supercoiled DNA by introducing transient single …
camptothecin, catalyzes the unwinding of supercoiled DNA by introducing transient single …