In vivo molecular bioluminescence imaging: new tools and applications
in vivo bioluminescence imaging (BLi) is an optical molecular imaging technique used to
visualize molecular and cellular processes in health and diseases and to follow the fate of …
visualize molecular and cellular processes in health and diseases and to follow the fate of …
Recent progress in CRISPR/Cas9 technology
Y Mei, Y Wang, H Chen, ZS Sun, XD Ju - Journal of Genetics and …, 2016 - Elsevier
The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system, a
simple and efficient tool for genome editing, has experienced rapid progress in its …
simple and efficient tool for genome editing, has experienced rapid progress in its …
The DUF1669 domain of FAM83 family proteins anchor casein kinase 1 isoforms
Members of the casein kinase 1 (CK1) family of serine-threonine protein kinases are
implicated in the regulation of many cellular processes, including the cell cycle, circadian …
implicated in the regulation of many cellular processes, including the cell cycle, circadian …
Customizable gene sensing and response without altering endogenous coding sequences
Synthetic biology aims to modify cellular behaviors by implementing genetic circuits that
respond to changes in cell state. Integrating genetic biosensors into endogenous gene …
respond to changes in cell state. Integrating genetic biosensors into endogenous gene …
An affinity-directed protein missile system for targeted proteolysis
The von Hippel–Lindau (VHL) protein serves to recruit the hypoxia-inducible factor alpha
(HIF1α) protein under normoxia to the CUL2 E3 ubiquitin ligase for its ubiquitylation and …
(HIF1α) protein under normoxia to the CUL2 E3 ubiquitin ligase for its ubiquitylation and …
Integral gene drives for population replacement
A Nash, GM Urdaneta, AK Beaghton… - Biology …, 2019 - journals.biologists.com
ABSTRACT A first generation of CRISPR-based gene drives has now been tested in the
laboratory in a number of organisms, including malaria vector mosquitoes. Challenges for …
laboratory in a number of organisms, including malaria vector mosquitoes. Challenges for …
Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time
CW White, HK Vanyai, HB See, EKM Johnstone… - Scientific Reports, 2017 - nature.com
Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding
G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein …
G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein …
Recent advances in therapeutic CRISPR-Cas9 genome editing: mechanisms and applications
L Zhou, S Yao - Molecular Biomedicine, 2023 - Springer
Recently, clustered regularly interspaced palindromic repeats (CRISPR)-Cas9 derived
editing tools had significantly improved our ability to make desired changes in the genome …
editing tools had significantly improved our ability to make desired changes in the genome …
FAM 83D directs protein kinase CK 1α to the mitotic spindle for proper spindle positioning
LJ Fulcher, Z He, L Mei, TJ Macartney, NT Wood… - EMBO …, 2019 - embopress.org
The concerted action of many protein kinases helps orchestrate the error‐free progression
through mitosis of mammalian cells. The roles and regulation of some prominent mitotic …
through mitosis of mammalian cells. The roles and regulation of some prominent mitotic …
Expansion of the CRISPR/Cas genome-sculpting toolbox: Innovations, applications and challenges
The emergence of the versatile gene-editing technology using programmable sequence-
specific endonuclease system (CRISPR-Cas9) has instigated a major upheaval in …
specific endonuclease system (CRISPR-Cas9) has instigated a major upheaval in …