In addition to biochemical signals and genetic considerations, mechanical forces are rapidly
emerging as a master regulator of human physiology. However, the molecular mechanisms …

The discovery of the giant protein titin, also known as connectin, dates almost half a century
back. In this review, I recapitulate major advances in the discovery of the titin filaments and …

Monitoring autophagic flux is necessary for most autophagy studies. The autophagic flux
assays currently available for mammalian cells are generally complicated and do not yield …

Skeletal muscle force production is increased at longer compared to shorter muscle lengths
because of length-dependent priming of thick filament proteins in the contractile unit before …

Myocardial passive stiffness is crucial for the heart's pump function and is determined by
mechanical elements, including the extracellular matrix and cytoskeletal filaments; however …

Muscles generate forces for animal locomotion. The contractile apparatus of muscles is the
sarcomere, a highly regular array of large actin and myosin filaments linked by gigantic titin …

Sarcomeres are the force-producing units of all striated muscles. Their nanoarchitecture
critically depends on the large titin protein, which in vertebrates spans from the sarcomeric Z …

Measuring the positions and dynamics of proteins in intact tissues or whole animals is key to
understanding protein function. However, to date, this is challenging, as the accessibility of …

How proteins respond to pulling forces, or protein nanomechanics, is a key contributor to the
form and function of biological systems. Indeed, the conventional view that proteins are able …

Muscle ankyrin repeat protein 1 (MARP1) is frequently up-regulated in stressed muscle, but
its effect on skeletal muscle function is poorly understood. Here, we focused on its …