Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …

The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

We report a comprehensive toolkit that enables targeted, specific modification of monocot
and dicot genomes using a variety of genome engineering approaches. Our reagents …

Targeted genome editing using artificial nucleases has the potential to accelerate basic
research as well as plant breeding by providing the means to modify genomes rapidly in a …

Background The use of homologous recombination to precisely modify plant genomes has
been challenging, due to the lack of efficient methods for delivering DNA repair templates to …

Background The ability to selectively alter genomic DNA sequences in vivo is a powerful tool
for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA …

The ability to edit plant genomes through gene targeting (GT) requires efficient methods to
deliver both sequence‐specific nucleases (SSN s) and repair templates to plant cells. This is …

With the rapid increase in the global population and the impact of climate change on
agriculture, there is a need for crops with higher yields and greater tolerance to abiotic …

Engineered nucleases can be used to induce site‐specific double‐strand breaks (DSB s) in
plant genomes. Thus, homologous recombination (HR) can be enhanced and targeted …

Genome editing promises giant leaps forward in advancing biotechnology, agriculture, and
basic research. The process relies on the use of sequence specific nucleases (SSNs) to …